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Your translated search: 4-nitroaniline = 794 reactions were found.
State Icon Reaction EC-Number Conf. maximal confidence score from EnzymeDetector PW
N-benzoyl-D-Arg_4-nitroanilide + H2O <=> benzoyl-D-Arg + 4-nitroaniline 3.4.22.40 bleomycin hydrolase
3.5.1.72 D-benzoylarginine-4-nitroanilide amidase


4-nitrophenyl-3-ketovalidamine <=> 4-nitroaniline + 5-D-(5/6)-5-C-(hydroxymethyl)-2,6-dihydroxycyclohex-2-en-1-one 4.3.3.1 3-ketovalidoxylamine C-N-lyase

5-D-glutamyl-4-nitroanilide + Gly-Gly <=> 4-nitroaniline + 5-L-glutamyl-Gly-Gly 2.3.2.2 gamma-glutamyltransferase

N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O <=> N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline 3.4.21.1 chymotrypsin
3.4.21.102 C-terminal processing peptidase
3.4.21.111 aqualysin 1
3.4.21.117 stratum corneum chymotryptic enzyme
3.4.21.12 alpha-lytic endopeptidase
3.4.21.2 chymotrypsin C
3.4.21.20 cathepsin G
3.4.21.37 leukocyte elastase
3.4.21.39 chymase
3.4.21.62 subtilisin
3.4.21.64 peptidase K
3.4.21.66 thermitase
3.4.21.77 semenogelase
3.4.21.80 streptogrisin A
3.4.21.81 streptogrisin B
3.4.21.96 lactocepin
3.4.21.B21
3.4.21.B3
3.4.21.B52
3.4.21.B54
3.4.21.B55
3.4.21.B57
3.4.24.72 fibrolase
3.4.24.B26
























phenylacetate_4-nitroanilide + H2O <=> phenylacetate + 4-nitroaniline 3.5.1.11 penicillin amidase

p-nitroacetanilide + H2O <=> 4-nitroaniline + acetate 3.5.1.13 aryl-acylamidase
3.5.1.4 amidase
3.5.1.41 chitin deacetylase



L-Leu-4-nitroanilide + H2O <=> L-leucine + 4-nitroaniline 3.3.2.6 leukotriene-A4 hydrolase
3.4.11.1 leucyl aminopeptidase
3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.13 clostridial aminopeptidase
3.4.11.14 cytosol alanyl aminopeptidase
3.4.11.15 aminopeptidase Y
3.4.11.2 membrane alanyl aminopeptidase
3.4.11.20 aminopeptidase Ey
3.4.11.22 aminopeptidase I
3.4.11.23 PepB aminopeptidase
3.4.11.24 aminopeptidase S
3.4.11.3 cystinyl aminopeptidase
3.4.11.5 prolyl aminopeptidase
3.4.11.6 aminopeptidase B
3.4.11.9 Xaa-Pro aminopeptidase
3.4.11.B3
3.4.11.B5
3.4.11.B6
3.4.11.B9
3.4.16.5 carboxypeptidase C
3.4.21.25 cucumisin
3.4.21.26 prolyl oligopeptidase
3.4.22.40 bleomycin hydrolase
3.4.23.4 chymosin
3.5.1.13 aryl-acylamidase

























L-Lys-4-nitroanilide + H2O <=> L-lysine + 4-nitroaniline 3.3.2.6 leukotriene-A4 hydrolase
3.4.11.1 leucyl aminopeptidase
3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.15 aminopeptidase Y
3.4.11.2 membrane alanyl aminopeptidase
3.4.11.20 aminopeptidase Ey
3.4.11.22 aminopeptidase I
3.4.11.24 aminopeptidase S
3.4.11.3 cystinyl aminopeptidase
3.4.11.4 tripeptide aminopeptidase
3.4.11.5 prolyl aminopeptidase
3.4.11.6 aminopeptidase B
3.4.11.B3
3.4.11.B9
3.4.13.20 beta-Ala-His dipeptidase
3.4.21.50 lysyl endopeptidase
















Met-4-nitroanilide + H2O <=> L-methionine + 4-nitroaniline 3.3.2.6 leukotriene-A4 hydrolase
3.4.11.1 leucyl aminopeptidase
3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.14 cytosol alanyl aminopeptidase
3.4.11.18 methionyl aminopeptidase
3.4.11.2 membrane alanyl aminopeptidase
3.4.11.20 aminopeptidase Ey
3.4.11.22 aminopeptidase I
3.4.11.24 aminopeptidase S
3.4.11.3 cystinyl aminopeptidase
3.4.11.6 aminopeptidase B
3.4.11.B3
3.4.11.B5
3.4.11.B9
3.4.21.26 prolyl oligopeptidase















L-Phe-4-nitroanilide + H2O <=> L-phenylalanine + 4-nitroaniline 3.4.11.1 leucyl aminopeptidase
3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.2 membrane alanyl aminopeptidase
3.4.11.20 aminopeptidase Ey
3.4.11.22 aminopeptidase I
3.4.11.24 aminopeptidase S
3.4.13.20 beta-Ala-His dipeptidase
3.4.14.5 dipeptidyl-peptidase IV
3.4.21.26 prolyl oligopeptidase
3.4.22.40 bleomycin hydrolase










Ala-4-nitroanilide + H2O <=> L-alanine + 4-nitroaniline 3.3.2.6 leukotriene-A4 hydrolase
3.4.11.1 leucyl aminopeptidase
3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.14 cytosol alanyl aminopeptidase
3.4.11.2 membrane alanyl aminopeptidase
3.4.11.20 aminopeptidase Ey
3.4.11.22 aminopeptidase I
3.4.11.24 aminopeptidase S
3.4.11.3 cystinyl aminopeptidase
3.4.11.5 prolyl aminopeptidase
3.4.11.6 aminopeptidase B
3.4.11.B3
3.4.11.B9
3.4.14.5 dipeptidyl-peptidase IV
3.4.19.1 acylaminoacyl-peptidase
3.4.21.25 cucumisin
3.4.22.40 bleomycin hydrolase

















L-Val-4-nitroanilide + H2O <=> L-valine + 4-nitroaniline 3.3.2.6 leukotriene-A4 hydrolase
3.4.11.1 leucyl aminopeptidase
3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.14 cytosol alanyl aminopeptidase
3.4.11.2 membrane alanyl aminopeptidase
3.4.11.20 aminopeptidase Ey
3.4.11.22 aminopeptidase I
3.4.11.24 aminopeptidase S
3.4.11.5 prolyl aminopeptidase
3.4.11.6 aminopeptidase B
3.4.11.B9











Gly-4-nitroanilide + H2O <=> glycine + 4-nitroaniline 3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.19 D-stereospecific aminopeptidase
3.4.11.2 membrane alanyl aminopeptidase
3.4.11.22 aminopeptidase I
3.4.11.24 aminopeptidase S
3.4.11.6 aminopeptidase B
3.4.11.B3
3.4.11.B9
3.4.13.20 beta-Ala-His dipeptidase
3.4.14.5 dipeptidyl-peptidase IV
3.4.22.25 glycyl endopeptidase











Pro-p-nitroanilide + H2O <=> L-proline + 4-nitroaniline 3.3.2.6 leukotriene-A4 hydrolase
3.4.11.1 leucyl aminopeptidase
3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.14 cytosol alanyl aminopeptidase
3.4.11.15 aminopeptidase Y
3.4.11.2 membrane alanyl aminopeptidase
3.4.11.20 aminopeptidase Ey
3.4.11.22 aminopeptidase I
3.4.11.24 aminopeptidase S
3.4.11.5 prolyl aminopeptidase
3.4.11.B3
3.4.11.B9
3.4.14.5 dipeptidyl-peptidase IV
3.4.21.26 prolyl oligopeptidase














benzoyl-L-arginine-p-nitroanilide + H2O <=> benzoyl-L-Arg + 4-nitroaniline 3.4.21.10 acrosin
3.4.21.27 coagulation factor XIa
3.4.21.4 trypsin
3.4.21.5 thrombin
3.4.21.57 leucyl endopeptidase
3.4.21.69 protein C (activated)
3.4.21.74 venombin A
3.4.21.91 flavivirin
3.4.21.B7
3.4.22.1 cathepsin B
3.4.22.14 actinidain
3.4.22.2 papain
3.4.22.3 ficain
3.4.22.30 caricain
3.4.22.37 gingipain R
3.4.22.7 asclepain
3.4.22.8 clostripain
3.4.24.35 gelatinase B


















N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O <=> N-succinyl-Ala-Ala-Ala + 4-nitroaniline 3.4.19.1 acylaminoacyl-peptidase
3.4.21.1 chymotrypsin
3.4.21.111 aqualysin 1
3.4.21.12 alpha-lytic endopeptidase
3.4.21.25 cucumisin
3.4.21.26 prolyl oligopeptidase
3.4.21.36 pancreatic elastase
3.4.21.37 leukocyte elastase
3.4.21.64 peptidase K
3.4.21.70 pancreatic endopeptidase E
3.4.22.16 cathepsin H
3.4.24.21 astacin
3.4.24.26 pseudolysin
3.4.24.B26














benzoyl-L-Pro-L-Phe-L-Arg-4-nitroanilide + H2O <=> Benzoyl-Pro-Phe-Arg + 4-nitroaniline 3.4.21.34 plasma kallikrein
3.4.21.35 tissue kallikrein
3.4.21.4 trypsin
3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.74 venombin A
3.4.21.83 oligopeptidase B
3.4.22.1 cathepsin B
3.4.22.37 gingipain R
3.4.22.48 staphopain
3.4.22.51 cruzipain










benzoyl-Arg-4-nitroanilide + H2O <=> benzoyl-DL-Arg + 4-nitroaniline 3.4.21.10 acrosin
3.4.21.4 trypsin
3.4.21.59 tryptase
3.4.21.62 subtilisin
3.4.21.74 venombin A
3.4.21.77 semenogelase
3.4.21.83 oligopeptidase B
3.4.21.9 enteropeptidase
3.4.22.2 papain
3.4.22.3 ficain
3.4.22.32 stem bromelain
3.4.22.33 fruit bromelain
3.4.22.37 gingipain R
3.4.22.6 chymopapain
3.4.22.8 clostripain
3.4.24.40 serralysin
3.4.24.72 fibrolase

















5-L-glutamyl-4-nitroanilide + H2O <=> 4-nitroaniline + L-glutamate 2.3.2.2 gamma-glutamyltransferase
3.2.1.17 lysozyme
3.4.11.10 bacterial leucyl aminopeptidase
3.4.19.13 glutathione gamma-glutamate hydrolase
3.4.19.3 pyroglutamyl-peptidase I
3.4.19.9 folate gamma-glutamyl hydrolase
3.4.21.25 cucumisin
3.5.1.93 glutaryl-7-aminocephalosporanic-acid acylase








D-Ala-4-nitroanilide + H2O <=> D-alanine + 4-nitroaniline 3.4.11.19 D-stereospecific aminopeptidase
3.4.13.20 beta-Ala-His dipeptidase


Ala-Pro-p-nitroanilide + H2O <=> Ala-Pro + 4-nitroaniline 3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.9 Xaa-Pro aminopeptidase
3.4.13.18 cytosol nonspecific dipeptidase
3.4.14.11 Xaa-Pro dipeptidyl-peptidase
3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase
3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV
3.4.16.2 lysosomal Pro-Xaa carboxypeptidase
3.4.21.26 prolyl oligopeptidase









Arg-Pro-4-nitroanilide + H2O <=> Arg-Pro + 4-nitroaniline 3.4.14.11 Xaa-Pro dipeptidyl-peptidase
3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV
3.4.21.26 prolyl oligopeptidase




Gly-Pro-4-nitroanilide + H2O <=> Gly-Pro + 4-nitroaniline 3.4.13.18 cytosol nonspecific dipeptidase
3.4.14.11 Xaa-Pro dipeptidyl-peptidase
3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase
3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV
3.4.16.2 lysosomal Pro-Xaa carboxypeptidase
3.4.21.26 prolyl oligopeptidase







Ala-Ala-4-nitroanilide + H2O <=> Ala-Ala + 4-nitroaniline 3.4.11.6 aminopeptidase B
3.4.11.B9
3.4.13.18 cytosol nonspecific dipeptidase
3.4.14.11 Xaa-Pro dipeptidyl-peptidase
3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV
3.4.21.25 cucumisin







Lys-p-nitroanilide + H2O <=> L-lysine + 4-nitroaniline 3.4.11.14 cytosol alanyl aminopeptidase
3.4.11.2 membrane alanyl aminopeptidase
3.4.21.50 lysyl endopeptidase
3.4.22.40 bleomycin hydrolase




TRPIITTA-p-nitroanilide + H2O <=> TRPIITTA + 4-nitroaniline 3.4.22.29 picornain 2A

Glu-p-nitroanilide + H2O <=> L-glutamate + 4-nitroaniline 3.4.11.21 aspartyl aminopeptidase
3.4.11.7 glutamyl aminopeptidase


Asp-Pro-p-nitroanilide + H2O <=> Asp-Pro + 4-nitroaniline 3.4.13.18 cytosol nonspecific dipeptidase
3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV
3.4.21.26 prolyl oligopeptidase




L-pyroglutamyl-p-nitroanilide + H2O <=> 5-oxoproline + 4-nitroaniline 3.4.19.3 pyroglutamyl-peptidase I

succinyl-Ala-Ala-Pro-Met-4-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Met + 4-nitroaniline 3.4.21.1 chymotrypsin
3.4.21.2 chymotrypsin C
3.4.21.62 subtilisin
3.4.21.B55




benzyloxycarbonyl-Ala-Ala-p-nitroanilide + H2O <=> Benzyloxycarbonyl-Ala-Ala + 4-nitroaniline 3.4.21.26 prolyl oligopeptidase
3.4.21.62 subtilisin


succinyl-Ala-Ala-Pro-Ala-p-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Ala + 4-nitroaniline 3.4.21.62 subtilisin

Glu-Gly-Arg-p-nitroanilide + H2O <=> Glu-Gly-Arg + 4-nitroaniline 3.4.21.4 trypsin
3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.68 t-plasminogen activator



tosyl-Gly-Pro-Lys-p-nitroanilide + H2O <=> Tosyl-Gly-Pro-Lys + 4-nitroaniline 3.4.21.50 lysyl endopeptidase
3.4.21.59 tryptase
3.4.21.89 signal peptidase I
3.4.21.B3




Z-D-Arg-Gly-Arg-p-nitroanilide + H2O <=> Z-D-Arg-Gly-Arg + 4-nitroaniline 3.4.21.59 tryptase
3.4.21.6 coagulation factor Xa
3.4.21.B1



benzyloxycarbonyl-L-Arg-L-Arg-4-nitroanilide + H2O <=> Benzyloxycarbonyl-Arg-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_
3.4.22.1 cathepsin B
3.4.22.26 cancer procoagulant
3.4.22.32 stem bromelain
3.4.22.33 fruit bromelain
3.4.22.35 histolysain
3.4.22.37 gingipain R
3.4.22.B6








benzyloxycarbonyl-Phe-Arg-4-nitroanilide + H2O <=> Benzyloxycarbonyl-Phe-Arg + 4-nitroaniline 3.4.22.1 cathepsin B
3.4.22.2 papain
3.4.22.B49
3.4.22.B5




acetyl-L-Leu-L-Leu-L-Arg-4-nitroanilide + H2O <=> acetyl-Leu-Leu-Arg + 4-nitroaniline 3.4.21.83 oligopeptidase B
3.4.21.B3


alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg-4-nitroanilide + H2O <=> alpha-N-benzyloxycarbonyl-Ala-Ala-Ala-Arg + 4-nitroaniline 3.4.21.B3

alpha-N-benzyloxycarbonyl-Ala-Ala-Ile-Arg-Arg-4-nitroanilide + H2O <=> alpha-N-benzyloxycarbonyl-Ala-Ala-Ile-Arg-Arg + 4-nitroaniline 3.4.21.B3

alpha-N-succinyl-Ala-Ala-Phe-4-nitroanilide + H2O <=> Succinyl-Ala-Ala-Phe + 4-nitroaniline 3.4.21.B3

N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O <=> N-methoxysuccinyl-Ala-Ala-Pro-Val + 4-nitroaniline 3.4.21.37 leukocyte elastase
3.4.21.62 subtilisin
3.4.21.76 myeloblastin



Met-Leu-p-nitroanilide + H2O <=> Met-Leu + 4-nitroaniline 3.4.11.10 bacterial leucyl aminopeptidase

arginine-4-nitroanilide + H2O <=> L-arginine + 4-nitroaniline 3.4.11.3 cystinyl aminopeptidase
3.4.11.6 aminopeptidase B
3.4.22.3 ficain
3.4.22.40 bleomycin hydrolase




H-Asp-p-nitroanilide + H2O <=> 4-nitroaniline + L-aspartate 3.5.1.26 N4-(beta-N-acetylglucosaminyl)-L-asparaginase

succinyl-Ala-Ala-Pro-Glu-p-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

5''-phospho-pyridoxyl-4-aminonitrobenzene + H2O + O2 <=> pyridoxal_5''-phosphate + 4-nitroaniline + H2O2 1.4.3.5 pyridoxal 5'-phosphate synthase

acetyl-CoA + 4-nitroaniline <=> CoA + p-nitroacetanilide 2.3.1.5 arylamine N-acetyltransferase

p-nitroacetanilide + H2O <=> 4-nitroaniline + NH3 3.5.1.4 amidase

L-Glu-4-nitroanilide + H2O <=> L-glutamate + 4-nitroaniline 3.4.11.1 leucyl aminopeptidase
3.4.11.15 aminopeptidase Y
3.4.11.2 membrane alanyl aminopeptidase
3.4.11.21 aspartyl aminopeptidase
3.4.11.22 aminopeptidase I
3.4.11.24 aminopeptidase S
3.4.11.7 glutamyl aminopeptidase
3.4.19.13 glutathione gamma-glutamate hydrolase
3.5.1.2 glutaminase









5-D-glutamyl-4-nitroanilide + H2O <=> L-glutamate + 4-nitroaniline 2.3.2.9 agaritine gamma-glutamyltransferase
3.4.19.13 glutathione gamma-glutamate hydrolase


1-(4''-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene + NADH <=> 4-nitroaniline + NAD+ + 1-amino-2-hydroxy-6-sulfonaphthalene 1.7.1.6 azobenzene reductase

5-L-glutamyl-4-nitroanilide + Cys-Gly <=> 4-nitroaniline + glutathione 2.3.2.2 gamma-glutamyltransferase

L-glutamine-4-nitroanilide + H2O <=> L-glutamate + 4-nitroaniline 3.4.11.6 aminopeptidase B

1-(4''-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene + NADPH <=> 4-nitroaniline + NADP+ + 1-amino-2-hydroxy-6-sulfonaphthalene 1.7.1.6 azobenzene reductase

Gly-p-nitroanilide + H2O <=> glycine + 4-nitroaniline 3.4.11.20 aminopeptidase Ey
3.4.11.5 prolyl aminopeptidase
3.4.22.40 bleomycin hydrolase
3.5.1.13 aryl-acylamidase




L-cystinyl-p-nitroanilide + H2O <=> L-cysteine + 4-nitroaniline 3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.3 cystinyl aminopeptidase


malonyl-CoA + 4-nitroaniline <=> CoA + N-(4-nitrophenyl)-malonamate 2.3.1.114 3,4-dichloroaniline N-malonyltransferase

Met-paranitroanilide + H2O <=> L-methionine + 4-nitroaniline 3.4.22.40 bleomycin hydrolase

Gly-Pro-4-nitroanilide + H2O <=> glycine + L-proline + 4-nitroaniline 3.4.13.9 Xaa-Pro dipeptidase

L-Ser-4-nitroanilide + H2O <=> L-serine + 4-nitroaniline 3.4.11.2 membrane alanyl aminopeptidase

Asp-p-nitroanilide + H2O <=> L-aspartate + 4-nitroaniline 3.4.11.21 aspartyl aminopeptidase
3.4.22.40 bleomycin hydrolase
3.5.1.13 aryl-acylamidase



L-aspartic_acid-p-nitrolanilide + H2O <=> 4-nitroaniline + L-aspartate 3.5.1.26 N4-(beta-N-acetylglucosaminyl)-L-asparaginase

L-Asp-4-nitroanilide + H2O <=> L-aspartate + 4-nitroaniline 3.4.11.2 membrane alanyl aminopeptidase
3.4.11.21 aspartyl aminopeptidase
3.4.11.22 aminopeptidase I
3.4.11.7 glutamyl aminopeptidase
3.4.13.21 dipeptidase E





N-tert-butyloxycarbonyl-Ala-Ala-p-nitroanilide + H2O <=> Nalpha-(tert-butoxycarbonyl)-L-alanyl-L-alanine + 4-nitroaniline 3.4.21.4 trypsin

peptide-4-nitroanilide + H2O <=> peptide + 4-nitroaniline 3.4.21.86 limulus clotting enzyme

peptidyl-p-nitroanilide + H2O <=> 4-nitroaniline + peptide 3.4.21.25 cucumisin

succinyl-Glu-Ala-Pro-Phe-4-nitroanilide + H2O <=> succinyl-Glu-Ala-Pro-Phe + 4-nitroaniline 3.4.21.B55

succinyl-Lys-Ala-Pro-Phe-4-nitroanilide + H2O <=> succinyl-Lys-Ala-Pro-Phe + 4-nitroaniline 3.4.21.B55

L-Tyr-4-nitroanilide + H2O <=> L-tyrosine + 4-nitroaniline 3.4.11.2 membrane alanyl aminopeptidase
3.4.21.26 prolyl oligopeptidase
3.4.22.40 bleomycin hydrolase



5-L-glutamyl-4-nitroanilide + L-leucine <=> 4-nitroaniline + 5-L-glutamyl-L-leucine 2.3.2.2 gamma-glutamyltransferase

Z-Gly-Pro-Arg-p-nitroanilide + H2O <=> Benzyloxycarbonyl-Gly-Pro-Arg + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme
3.4.21.4 trypsin
3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.59 tryptase
3.4.21.9 enteropeptidase





5-L-glutamyl-4-nitroanilide + ethylamine <=> Theanine + 4-nitroaniline 2.3.2.2 gamma-glutamyltransferase

N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O <=> Tosyl-Gly-Pro-Lys + 4-nitroaniline 3.4.11.6 aminopeptidase B
3.4.21.4 trypsin
3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.50 lysyl endopeptidase
3.4.21.59 tryptase
3.4.21.7 plasmin
3.4.21.74 venombin A
3.4.21.B3
3.4.22.26 cancer procoagulant
3.4.22.47 gingipain K
3.4.24.B39











Gly-Pro-Arg-p-nitroanilide + H2O <=> Gly-Pro-Arg + 4-nitroaniline 3.4.21.4 trypsin
3.4.22.38 cathepsin K


benzyloxycarbonyl-GGL-4-nitroanilide + H2O <=> benzyloxycarbonyl-Gly-Gly-Leu + 4-nitroaniline 3.4.19.1 acylaminoacyl-peptidase

GDDDDK-4-nitroanilide + H2O <=> Gly-Asp-Asp-Asp-Asp-Lys + 4-nitroaniline 3.4.21.9 enteropeptidase

Val-Ala-4-nitroanilide + H2O <=> Val-Ala + 4-nitroaniline 3.4.14.2 dipeptidyl-peptidase II

acetyl-Ala-Phe-Arg-p-nitroanilide + H2O <=> acetyl-Ala-Phe-Arg + 4-nitroaniline 3.4.21.35 tissue kallikrein

acetyl-IETD-4-nitroanilide + H2O <=> acetyl-IETD + 4-nitroaniline 3.4.22.61 caspase-8

acetyl-IETD-p-nitroanilide + H2O <=> acetyl-IETD + 4-nitroaniline 3.4.22.61 caspase-8

acetyl-LEHD-p-nitroanilide + H2O <=> acetyl-LEHD + 4-nitroaniline 3.4.22.56 caspase-3
3.4.22.61 caspase-8
3.4.22.62 caspase-9



PMMGKASPV-4-nitroanilide + H2O <=> PMMGKASPV + 4-nitroaniline 3.4.21.107 peptidase Do

PVFNTLPMMGKASPV-4-nitroanilide + H2O <=> PVFNTLPMMGKASPV + 4-nitroaniline 3.4.21.107 peptidase Do

SPMFKGV-para-nitroaniline + H2O <=> SPMFKGV + 4-nitroaniline 3.4.21.107 peptidase Do

VFNTLPMMGKASPV-4-nitroanilide + H2O <=> VFNTLPMMGKASPV + 4-nitroaniline 3.4.21.107 peptidase Do

Ac-FM(O2)YK-4-nitroanilide + H2O <=> Ac-FM(O2)YK + 4-nitroaniline 3.4.21.7 plasmin

Ac-KM(O2)FR-4-nitroanilide + H2O <=> Ac-KM(O2)FR + 4-nitroaniline 3.4.21.7 plasmin

Ac-KM(O2)YR-4-nitroanilide + H2O <=> Ac-KM(O2)YR + 4-nitroaniline 3.4.21.7 plasmin

Ac-LEDH-4-nitroanilide + H2O <=> Ac-LEDH + 4-nitroaniline 3.4.22.62 caspase-9

Ac-Lys-Arg-4-nitroanilide + H2O <=> Ac-Lys-Arg + 4-nitroaniline 3.4.21.46 complement factor D

Ac-RM(O2)WR-4-nitroanilide + H2O <=> Ac-RM(O2)WR + 4-nitroaniline 3.4.21.7 plasmin

Ac-RM(O2)YR-4-nitroanilide + H2O <=> Ac-RM(O2)YR + 4-nitroaniline 3.4.21.7 plasmin

acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide + H2O <=> acetyl-L-Leu-L-Lys-L-Arg + 4-nitroaniline 3.4.21.83 oligopeptidase B

benzoyl-beta-Ala-Gly-Arg-4-nitroanilide + H2O <=> benzoyl-beta-Ala-Gly-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.73 u-plasminogen activator


benzyloxycarbonyl-Val-Pro-Arg-4-nitroanilide + H2O <=> benzyloxycarbonyl-Val-Pro-Arg + 4-nitroaniline 3.4.22.26 cancer procoagulant

C2H5CO-Lys-(epsilon-Cbz)-Gly-Arg-4-nitroanilide + H2O <=> C2H5CO-Lys-(epsilon-Cbz)-Gly-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

KQWK-4-nitroanilide + H2O <=> KQWK + 4-nitroaniline 3.4.21.7 plasmin

KTFK-4-nitroanilide + H2O <=> KTFK + 4-nitroaniline 3.4.21.7 plasmin

p-nitroacetanilide + aniline <=> 4-nitroaniline + acetanilide 2.3.1.5 arylamine N-acetyltransferase

DPMFKLV-4-nitroanilide + H2O <=> DPMFKLV + 4-nitroaniline 3.4.21.107 peptidase Do

L-Asp-L-Ala-L-Ala-L-Arg-4-nitroanilide + H2O <=> L-Asp-L-Ala-L-Ala-L-Arg + 4-nitroaniline 3.4.21.106 hepsin

L-Asp-L-Lys-(gamma-Cbo)-L-Pro-L-Arg-4-nitroanilide + H2O <=> L-Asp-L-Lys-(gamma-Cbo)-L-Pro-L-Arg + 4-nitroaniline 3.4.21.106 hepsin

L-Leu-Gly-L-Arg-4-nitroanilide + H2O <=> L-Leu-Gly-L-Arg + 4-nitroaniline 3.4.21.22 coagulation factor IXa

L-Phe-L-Leu-L-Glu-4-nitroanilide + H2O <=> L-Phe-L-Leu-L-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

L-pyro-Glu-Pro-Arg-4-nitroanilide + H2O <=> L-pyro-Glu-Pro-Arg + 4-nitroaniline 3.4.21.27 coagulation factor XIa

L-pyroGlu-L-Pro-L-Arg-4-nitroanilide + H2O <=> L-pyro-Glu-Pro-Arg + 4-nitroaniline 3.4.21.27 coagulation factor XIa

LEDH-4-nitroanilide + H2O <=> LEDH + 4-nitroaniline 3.4.22.62 caspase-9

N-acetyl-YRFK-4-nitroanilide + H2O <=> N-acetyl-YRFK + 4-nitroaniline 3.4.21.B4

N-succinyl-L-Phe-L-Pro-L-Phe-4-nitroanilide + H2O <=> N-succinyl-L-Phe-L-Pro-L-Phe + 4-nitroaniline 3.4.21.20 cathepsin G

RQWK-4-nitroanilide + H2O <=> RQWK + 4-nitroaniline 3.4.21.7 plasmin

succinyl-L-Ala-L-Ala-L-Pro-L-Asp-4-nitroanilide + H2O <=> succinyl-L-Ala-L-Ala-L-Pro-L-Asp + 4-nitroaniline 3.4.21.81 streptogrisin B

VQYK-4-nitroanilide + H2O <=> VQYK + 4-nitroaniline 3.4.21.7 plasmin

VQYR-4-nitroanilide + H2O <=> VQYR + 4-nitroaniline 3.4.21.7 plasmin

acetyl-L-Leu-L-Lys-L-Arg-4-nitroanilide + H2O <=> alpha-N-acetyl-Leu-Lys + L-arginine + 4-nitroaniline 3.4.21.B3

Suc-AAA-4-nitroanilide + H2O <=> N-succinyl-Ala-Ala-Ala + 4-nitroaniline 3.4.21.76 myeloblastin

succinyl-Ala-Ala-Ala-p-nitroanilide + H2O <=> N-succinyl-Ala-Ala-Ala + 4-nitroaniline 3.4.21.36 pancreatic elastase
3.4.21.37 leukocyte elastase
3.4.21.62 subtilisin
3.4.21.64 peptidase K




Arg-p-nitroanilide + H2O <=> L-arginine + 4-nitroaniline 3.4.22.40 bleomycin hydrolase

L-Arg-p-nitroanilide + H2O <=> L-arginine + 4-nitroaniline 3.3.2.6 leukotriene-A4 hydrolase
3.4.11.1 leucyl aminopeptidase
3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.15 aminopeptidase Y
3.4.11.2 membrane alanyl aminopeptidase
3.4.11.20 aminopeptidase Ey
3.4.11.22 aminopeptidase I
3.4.11.24 aminopeptidase S
3.4.11.3 cystinyl aminopeptidase
3.4.11.6 aminopeptidase B
3.4.11.B3
3.4.11.B9
3.4.13.20 beta-Ala-His dipeptidase
3.4.14.5 dipeptidyl-peptidase IV
3.4.21.4 trypsin















acetyl-Ile-Glu-Thr-Asp-p-nitroanilide + H2O <=> Ac-IETD + 4-nitroaniline 3.4.22.56 caspase-3

N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide + H2O <=> 4-nitroaniline + Ac-DEVD 3.4.22.56 caspase-3
3.4.22.60 caspase-7


RQFR-4-nitroanilide + H2O <=> RQFR + 4-nitroaniline 3.4.21.7 plasmin

Ala-Ala-Pro-Phe-4-nitroanilide + H2O <=> L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline 3.4.21.39 chymase
3.4.21.5 thrombin
3.4.21.62 subtilisin
3.4.21.63 oryzin




Ala-Ala-Val-Ala-4-nitroanilide + H2O <=> L-Ala-L-Ala-L-Val-L-Ala + 4-nitroaniline 3.4.21.62 subtilisin

Phe-Ala-Ala-Phe-4-nitroanilide + H2O <=> L-Phe-L-Ala-L-Ala-L-Phe + 4-nitroaniline 3.4.21.62 subtilisin

YLSR-4-nitroanilide + H2O <=> YLSR + 4-nitroaniline 3.4.21.B45

YLVR-4-nitroanilide + H2O <=> YLVR + 4-nitroaniline 3.4.21.B45

YVNR-4-nitroanilide + H2O <=> YVNR + 4-nitroaniline 3.4.21.B45

YVSR-4-nitroanilide + H2O <=> YVSR + 4-nitroaniline 3.4.21.B45

YVVR-4-nitroanilide + H2O <=> YVVR + 4-nitroaniline 3.4.21.B45

Z-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O <=> Z-L-Ala-L-Ala-L-Ala-L-Pro + 4-nitroaniline 3.4.21.26 prolyl oligopeptidase

N-succinyl-L-Leu-4-nitroanilide + H2O <=> N-Succinyl-L-Leu + 4-nitroaniline 3.4.11.1 leucyl aminopeptidase

AcG-Y-betaA-R-R-R-A-R-R-R-R-V-L-R-4-nitroanilide + H2O <=> AcG-Y-betaA-R-R-R-A-R-R-R-R-V-L-R + 4-nitroaniline 3.4.24.83 anthrax lethal factor endopeptidase

methanesulfonyl-D-cyclohexylalanyl-butyl-arginine_p-nitroanilide + H2O <=> methanesulfonyl-D-cyclohexylalanyl-butyl-arginine + 4-nitroaniline 3.4.21.21 coagulation factor VIIa

L-Ala-L-Ala-L-Ala_4-nitroanilide + H2O <=> Ala-Ala-Ala + 4-nitroaniline 3.4.14.10 tripeptidyl-peptidase II

Ala-Ala-Ala_4-nitroanilide + H2O <=> Ala-Ala-Ala + 4-nitroaniline 3.4.11.B9
3.4.14.10 tripeptidyl-peptidase II
3.4.14.9 tripeptidyl-peptidase I



AEVD-4-nitroanilide + H2O <=> AEVD + 4-nitroaniline 3.4.22.63 caspase-10

Ala-Ala-Pro-Lys-4-nitroanilide + H2O <=> Ala-Ala-Pro-Lys + 4-nitroaniline 3.4.21.62 subtilisin

Ala-Ala-Pro-Nle-4-nitroanilide + H2O <=> Ala-Ala-Pro-Nle + 4-nitroaniline 3.4.21.62 subtilisin

2 beta-Ala-p-nitroanilide <=> beta-homoGly-beta-homoGly_4-nitroanilide + 4-nitroaniline 3.4.11.25 beta-peptidyl aminopeptidase

D-cyclohexyl-Gly-L-Pro-L-Arg-4-nitroanilide + H2O <=> D-cyclohexyl-Gly-L-Pro-L-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.6 coagulation factor Xa


N6-CBZ-D-Lys-L-Pro-L-Arg-4-nitroanilide + H2O <=> N6-CBZ-D-Lys-L-Pro-L-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.6 coagulation factor Xa


D-Leu-Phe-Gly-Arg-4-nitroanilide + H2O <=> D-Leu-Phe-Gly-Arg + 4-nitroaniline 3.4.21.22 coagulation factor IXa

D-Phe-Pip-Arg-p-nitroanilide + H2O <=> D-Phe-piperocolyl-L-Arg + 4-nitroaniline 3.4.21.59 tryptase
3.4.21.6 coagulation factor Xa


D-Phe-L-pipecolyl-L-Arg-p-nitroanilide + H2O <=> D-Phe-piperocolyl-L-Arg + 4-nitroaniline 3.4.21.21 coagulation factor VIIa

D-Leu-4-nitroanilide + H2O <=> D-Leu + 4-nitroaniline 3.4.13.22 D-Ala-D-Ala dipeptidase

decanoyl_p-nitroanilide + H2O <=> Decanoate + 4-nitroaniline 3.5.1.99 fatty acid amide hydrolase

HANR-4-nitroanilide + H2O <=> HANR + 4-nitroaniline 3.4.21.B45

HASR-4-nitroanilide + H2O <=> His-Ala-Ser-Arg + 4-nitroaniline 3.4.21.B45

HAVR-4-nitroanilide + H2O <=> HAVR + 4-nitroaniline 3.4.21.B45

HLNR-4-nitroanilide + H2O <=> HLNR + 4-nitroaniline 3.4.21.B45

HLSR-4-nitroanilide + H2O <=> HLSR + 4-nitroaniline 3.4.21.B45

beta-homoAla-p-nitroanilide + H2O <=> beta-homoAla + 4-nitroaniline 3.4.11.25 beta-peptidyl aminopeptidase
3.4.13.20 beta-Ala-His dipeptidase


HLVR-4-nitroanilide + H2O <=> HLVR + 4-nitroaniline 3.4.21.B45

HVNR-4-nitroanilide + H2O <=> HVNR + 4-nitroaniline 3.4.21.B45

HVSR-4-nitroanilide + H2O <=> HVSR + 4-nitroaniline 3.4.21.B45

HVVR-4-nitroanilide + H2O <=> HVVR + 4-nitroaniline 3.4.21.B45

beta-homoPhe-p-nitroanilide + H2O <=> beta-homoPhe + 4-nitroaniline 3.4.11.25 beta-peptidyl aminopeptidase

beta-homoPhe-4-nitroanilide + H2O <=> beta-homoPhe + 4-nitroaniline 3.4.13.20 beta-Ala-His dipeptidase

MANR-4-nitroanilide + H2O <=> MANR + 4-nitroaniline 3.4.21.B45

MASR-4-nitroanilide + H2O <=> MASR + 4-nitroaniline 3.4.21.B45

MAVR-4-nitroanilide + H2O <=> MAVR + 4-nitroaniline 3.4.21.B45

MLNR-4-nitroanilide + H2O <=> MLNR + 4-nitroaniline 3.4.21.B45

MLSR-4-nitroanilide + H2O <=> MLSR + 4-nitroaniline 3.4.21.B45

MLVR-4-nitroanilide + H2O <=> MLVR + 4-nitroaniline 3.4.21.B45

MVNR-4-nitroanilide + H2O <=> MVNR + 4-nitroaniline 3.4.21.B45

MVSR-4-nitroanilide + H2O <=> MVSR + 4-nitroaniline 3.4.21.B45

MVVR-4-nitroanilide + H2O <=> MVVR + 4-nitroaniline 3.4.21.B45

Ac-DEFQLQ-4-nitroanilide + H2O <=> N-acetyl-DEFQLQ + 4-nitroaniline 3.4.22.28 picornain 3C

N-benzyloxycarbonyl-L-Ala-L-Ala-L-Pro_4-nitroanilide + H2O <=> N-benzyloxycarbonyl-L-Ala-L-Ala-L-Pro + 4-nitroaniline 3.4.16.2 lysosomal Pro-Xaa carboxypeptidase

Z-Ala-Trp-Arg-nitroanilide + H2O <=> Z-Ala-Trp-Arg + 4-nitroaniline 3.4.21.9 enteropeptidase

N-succinyl-AAPN-4-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Asn + 4-nitroaniline 3.4.21.2 chymotrypsin C

succinyl-Ala-Ala-Pro-Gln_4-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Gln + 4-nitroaniline 3.4.21.2 chymotrypsin C

succinyl-Ala-Ala-Pro-Ile-4-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Ile + 4-nitroaniline 3.4.21.2 chymotrypsin C

VGSLR-4-nitroanilide + H2O <=> VGSLR + 4-nitroaniline 3.4.21.B45

WANR-4-nitroanilide + H2O <=> WANR + 4-nitroaniline 3.4.21.B45

WASR-4-nitroanilide + H2O <=> WASR + 4-nitroaniline 3.4.21.B45

WAVR-4-nitroanilide + H2O <=> WAVR + 4-nitroaniline 3.4.21.B45

WLNR-4-nitroanilide + H2O <=> WLNR + 4-nitroaniline 3.4.21.B45

WLSR-4-nitroanilide + H2O <=> WLSR + 4-nitroaniline 3.4.21.B45

WLVR-4-nitroanilide + H2O <=> WLVR + 4-nitroaniline 3.4.21.B45

WVNR-4-nitroanilide + H2O <=> WVNR + 4-nitroaniline 3.4.21.B45

WVSR-4-nitroanilide + H2O <=> WVSR + 4-nitroaniline 3.4.21.B45

WVVR-4-nitroanilide + H2O <=> WVVR + 4-nitroaniline 3.4.21.B45

YAAR-4-nitroanilide + H2O <=> YAAR + 4-nitroaniline 3.4.21.B45

YANR-4-nitroanilide + H2O <=> YANR + 4-nitroaniline 3.4.21.B45

YASR-4-nitroanilide + H2O <=> YASR + 4-nitroaniline 3.4.21.B45

YAVR-4-nitroanilide + H2O <=> YAVR + 4-nitroaniline 3.4.21.B45

YLNR-4-nitroanilide + H2O <=> YLNR + 4-nitroaniline 3.4.21.B45

benzyl-FVR-p-nitroanilide + H2O <=> benzyl-Phe-Val-Arg + 4-nitroaniline 3.4.22.2 papain
3.4.22.32 stem bromelain
3.4.22.33 fruit bromelain



FRLKGG-4-nitroanilide + H2O <=> FRLKGG + 4-nitroaniline 3.4.22.B50

benzyloxycarbonyl-Ala-Arg-Arg-4-nitroanilide + H2O <=> Benzyloxycarbonyl-Ala-Arg-Arg + 4-nitroaniline 3.4.22.35 histolysain

GSLR-4-nitroanilide + H2O <=> GSLR + 4-nitroaniline 3.4.21.B45

benzyloxycarbonyl-L-Lys-L-Arg_4-nitroanilide + H2O <=> Benzyloxycarbonyl-Lys-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

benzyloxycarbonyl-Val-Gly-Arg-p-nitroanilide + H2O <=> Benzyloxycarbonyl-Val-Gly-Arg + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme
3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.68 t-plasminogen activator



D-Phe-Pip-Arg-p-nitroanilide + H2O <=> D-Phe-pipecolyl-Arg + 4-nitroaniline 3.4.21.4 trypsin
3.4.21.5 thrombin
3.4.21.6 coagulation factor Xa
3.4.21.69 protein C (activated)
3.4.21.74 venombin A
3.4.21.B1
3.4.21.B7
3.4.22.26 cancer procoagulant
3.4.24.72 fibrolase









D-Phe-pipecolyl-Arg-p-nitroanilide + H2O <=> D-Phe-pipecolyl-Arg + 4-nitroaniline 3.4.21.21 coagulation factor VIIa

Phe-Pro-Ala-4-nitroanilide + H2O <=> Phe-Pro-Ala + 4-nitroaniline 3.4.14.9 tripeptidyl-peptidase I

Phe-Pro-Ala-p-nitroanilide + H2O <=> Phe-Pro-Ala + 4-nitroaniline 3.4.21.100 sedolisin

RKGDIKSY-p-nitroanilide + H2O <=> RKGDIKSY + 4-nitroaniline 3.4.22.29 picornain 2A

N-acetyl-L-Tyr-p-nitroanilide + H2O <=> N-acetyl-L-tyrosine + 4-nitroaniline 3.4.21.1 chymotrypsin

Acetyl-Tyr_4-nitroanilide + H2O <=> N-acetyl-L-tyrosine + 4-nitroaniline 3.4.19.1 acylaminoacyl-peptidase
3.4.21.83 oligopeptidase B


acetyl-Pro-Phe-Arg-p-nitroanilide + H2O <=> acetyl-Pro-Phe-Arg + 4-nitroaniline 3.4.21.118 kallikrein 8
3.4.21.35 tissue kallikrein


Glu-Pro-4-nitroanilide + H2O <=> Glu-Pro + 4-nitroaniline 3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV


acetyl-Ala-Leu-Arg-p-nitroanilide + H2O <=> acetyl-Ala-Leu-Arg + 4-nitroaniline 3.4.21.35 tissue kallikrein

N2-acetyl-N6-benzyloxycarbonyl-Lys-Gly-Arg_4-nitroanilide + H2O <=> N2-acetyl-N6-benzyloxycarbonyl-Lys-Gly-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

methoxysuccinyl-Val-4-nitroanilide <=> methoxysuccinyl-Val + 4-nitroaniline 3.4.21.37 leukocyte elastase

Suc-Tyr-Leu-Val-p-nitroanilide + H2O <=> 4-nitroaniline + Suc-Tyr-Leu-Val 3.4.21.71 pancreatic elastase II

D-Phe-L-pipecolyl-L-Arg-4-nitroanilide + H2O <=> D-Phe-L-pipecolyl-L-Arg + 4-nitroaniline 3.4.21.5 thrombin

L-Ala-L-Phe-L-Pro-4-nitroanilide + H2O <=> Ala-Phe-Pro + 4-nitroaniline 3.4.14.10 tripeptidyl-peptidase II
3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase


Ala-Phe-Pro-p-nitroanilide + H2O <=> Ala-Phe-Pro + 4-nitroaniline 3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase
3.4.14.9 tripeptidyl-peptidase I


Val-Pro-Arg_4-nitroanilide + H2O <=> Val-Pro-Arg + 4-nitroaniline 3.4.14.9 tripeptidyl-peptidase I
3.4.22.26 cancer procoagulant


methoxycarbonyl-D-cyclohexylglycyl-glycyl-L-arginyl-p-nitroanilide + H2O <=> methyloxycarbonyl-L-cyclohexylglycine-Gly-L-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

methoxycarbonyl-D-Nle-Gly-Arg-p-nitroanilide + H2O <=> methyloxycarbonyl-D-Nle-Gly-L-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

methyloxycarbonyl-D-L-hexahydrotyrosine-Gly-L-Arg_4-nitroaniline + H2O <=> methyloxycarbonyl-D-L-hexahydrotyrosine-Gly-L-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

D-Ile-L-Phe-L-Lys_4-nitroanilide + H2O <=> D-Ile-L-Phe-L-Lys + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

Suc-AAPL-4-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline 3.4.21.76 myeloblastin

Suc-Ala-Ala-Pro-Leu-p-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline 3.4.21.1 chymotrypsin
3.4.21.2 chymotrypsin C
3.4.21.20 cathepsin G
3.4.21.25 cucumisin
3.4.21.39 chymase
3.4.21.57 leucyl endopeptidase
3.4.21.62 subtilisin
3.4.21.64 peptidase K
3.4.21.81 streptogrisin B
3.4.21.B3
3.4.21.B57











succinyl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Leu + 4-nitroaniline 3.4.21.1 chymotrypsin
3.4.21.12 alpha-lytic endopeptidase
3.4.21.2 chymotrypsin C
3.4.21.62 subtilisin
3.4.21.B54
3.4.21.B57






benzyloxycarbonyl-Ala-Pro-p-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Pro + 4-nitroaniline 3.4.16.2 lysosomal Pro-Xaa carboxypeptidase
3.4.21.26 prolyl oligopeptidase


butyloxycarbonyl-O-benzoyl-L-Ser-Gly-Arg_4-nitroanilide + H2O <=> butyloxycarbonyl-O-benzoyl-L-Ser-Gly-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

butyloxycarbonyl-L-Leu-Gly-L-Arg_4-nitroanilide + H2O <=> butyloxycarbonyl-L-Leu-Gly-L-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

Benzyloxycarbonyl-Tyr-Lys-Arg_4-nitroanilide + H2O <=> benzyloxycarbonyl-L-Tyr-L-Lys-L-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

benzyloxycarbonyl-Gly-D-Ala-L-Arg_4-nitroanilide + H2O <=> benzyloxycarbonyl-Gly-D-Ala-L-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

Spectrozyme_PL + H2O <=> L-norleucyl-L-hexahydrotyrosyl-L-lysine + 4-nitroaniline 3.4.21.7 plasmin

DAP-4-nitroanilide + H2O <=> L-Asp-L-Ala-L-Pro + 4-nitroaniline 3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase

L-Ala-L-Pro-L-Pro-4-nitroanilide + H2O <=> L-Ala-L-Pro-L-Pro + 4-nitroaniline 3.4.21.26 prolyl oligopeptidase

KHLY-4-nitroanilide + H2O <=> KLHLY + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme

KHLF-4-nitroanilide + H2O <=> KLHLF + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme

benzyl-TAR-4-nitroanilide + H2O <=> benzyl-TAR + 4-nitroaniline 3.4.21.68 t-plasminogen activator

Ala-Ala-Pro-Asn-4-nitroanilide + H2O <=> Ala-Ala-Pro-Asn + 4-nitroaniline 3.4.21.63 oryzin

Ala-Ala-Pro-Arg-4-nitroanilide + H2O <=> Ala-Ala-Pro-Arg + 4-nitroaniline 3.4.21.63 oryzin

acetyl-QYAR-4-nitroanilide + H2O <=> acetyl-QYAR + 4-nitroaniline 3.4.21.68 t-plasminogen activator

acetyl-GTGR-4-nitroanilide + H2O <=> acetyl-GTGR + 4-nitroaniline 3.4.21.68 t-plasminogen activator

acetyl-GTAR-4-nitroanilide + H2O <=> acetyl-GTAR + 4-nitroaniline 3.4.21.68 t-plasminogen activator

acetyl-FYAR-4-nitroanilide + H2O <=> acetyl-FYAR + 4-nitroaniline 3.4.21.68 t-plasminogen activator

acetyl-FVQR-4-nitroanilide + H2O <=> acetyl-FVQR + 4-nitroaniline 3.4.21.B12

acetyl-FTAR-4-nitroanilide + H2O <=> acetyl-FTAR + 4-nitroaniline 3.4.21.68 t-plasminogen activator

QHLY-4-nitroanilide + H2O <=> QLHLY + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme

D-hexahydrotyrosyl-Gly-Arg-p-nitroanilide + H2O <=> D-hexahydrotyrosine-Gly-Arg + 4-nitroaniline 3.4.21.B60

Spectrozyme_FXIIa + H2O <=> D-cyclohexylalanine-Gly-Arg + 4-nitroaniline 3.4.21.B60

methylsulfonyl-D-cyclohexylglycyl-Gly-Arg-p-nitroanilide + H2O <=> CH3-SO2-D-cyclohexylglycine-Gly-Arg + 4-nitroaniline 3.4.21.B60

CH3-SO2-D-Arg-Gly-Arg-4-nitroanilide + H2O <=> CH3-SO2-D-Arg-Gly-Arg + 4-nitroaniline 3.4.21.B60

succinyl-L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O <=> succinyl-L-Ala-L-Ala-L-Ala-L-Pro + 4-nitroaniline 3.4.21.26 prolyl oligopeptidase

succinyl-L-Arg-L-Pro-4-nitroanilide + H2O <=> succinyl-L-Arg-L-Pro + 4-nitroaniline 3.4.21.26 prolyl oligopeptidase

heptanoyl_p-nitroanilide + H2O <=> Heptanoate + 4-nitroaniline 3.5.1.99 fatty acid amide hydrolase

succinyl-D-Asp-p-nitroanilide + H2O <=> succinyl-D-Asp + 4-nitroaniline 3.4.99.B2

glycine-L-phenylalanine-p-nitroanilide + H2O <=> Gly-Phe + 4-nitroaniline 3.4.14.1 dipeptidyl-peptidase I

Gly-Phe-4-nitroanilide + H2O <=> Gly-Phe + 4-nitroaniline 3.4.11.6 aminopeptidase B
3.4.14.1 dipeptidyl-peptidase I
3.4.14.5 dipeptidyl-peptidase IV



phenylacetyl-Lys-Lys-Arg-4-nitroanilide + H2O <=> phenylacetyl-Lys-Lys-Arg + 4-nitroaniline 3.4.21.91 flavivirin

N-(4-nitrophenyl)-6-aminohexanamide + H2O <=> 4-nitroaniline + 6-aminohexanoate 3.5.1.117 6-aminohexanoate-oligomer endohydrolase
3.5.1.46 6-aminohexanoate-oligomer exohydrolase


5-L-glutamyl-4-nitroanilide + L-glutamate <=> 4-nitroaniline + 5-L-glutamyl-L-glutamate 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-valine <=> 4-nitroaniline + 5-L-glutamyl-L-valine 2.3.2.2 gamma-glutamyltransferase

benzoyl-citrulline-p-nitroanilide + H2O <=> Benzoyl-citrulline + 4-nitroaniline 3.4.22.3 ficain

Benzoyl-citrulline_4-nitroanilide + H2O <=> Benzoyl-citrulline + 4-nitroaniline 3.4.22.32 stem bromelain

N-benzoyl-Pro-Phe-Arg-p-nitroanilide + H2O <=> Benzoyl-Pro-Phe-Arg + 4-nitroaniline 3.4.22.B5

N-benzoyl-Pro-Phe-Arg-4-nitroanilide + H2O <=> Benzoyl-Pro-Phe-Arg + 4-nitroaniline 3.4.21.34 plasma kallikrein
3.4.24.B39


benzyloxycarbonyl-Leu-Leu-Glu-p-nitroanilide + H2O <=> Benzyloxycarbonyl-Leu-Leu-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

Z-Leu-Leu-Glu-NH-4-nitroanilide + H2O <=> Benzyloxycarbonyl-Leu-Leu-Glu + 4-nitroaniline 3.4.22.40 bleomycin hydrolase

benzoyl-Arg-4-nitroanilide + H2O <=> benzoyl-L-Arg + 4-nitroaniline 3.4.11.6 aminopeptidase B
3.4.16.5 carboxypeptidase C
3.4.21.39 chymase
3.4.21.4 trypsin
3.4.21.48 cerevisin
3.4.21.54 gamma-renin
3.4.21.59 tryptase
3.4.21.74 venombin A
3.4.22.14 actinidain
3.4.22.15 cathepsin L
3.4.22.2 papain
3.4.22.37 gingipain R
3.4.22.8 clostripain













Gly-Pro-Met_4-nitroanilide + H2O <=> Gly-Pro-Met + 4-nitroaniline 3.4.14.9 tripeptidyl-peptidase I

Z-Ala-Ala-Arg-nitroanilide + H2O <=> 4-nitroaniline + N-Carbobenzoxy-Ala-Ala-Arg 3.4.21.83 oligopeptidase B
3.4.21.9 enteropeptidase


N-acetyl-L-phenylalanyl_4-nitroanilide + H2O <=> N-acetyl-L-phenylalanine + 4-nitroaniline 3.4.19.1 acylaminoacyl-peptidase

N-succinyl-Ala-Ala-Phe-4-nitroanilide + H2O <=> Succinyl-Ala-Ala-Phe + 4-nitroaniline 3.4.21.1 chymotrypsin
3.4.21.111 aqualysin 1
3.4.21.36 pancreatic elastase
3.4.21.62 subtilisin
3.4.21.B52





D-Val-L-cyclohexylalanine-L-Arg_4-nitroanilide + H2O <=> D-Val-cyclohexyl-Ala-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

acyl-L-lysine-4-nitroanilide + H2O <=> N6-acyl-L-lysine + 4-nitroaniline 3.4.22.8 clostripain

1,2-bis(4-fluorophenyl)ethane-1,2-dione + H2O <=> Benzyloxycarbonyl-Gly-Pro + 4-nitroaniline 3.4.16.2 lysosomal Pro-Xaa carboxypeptidase
3.4.21.26 prolyl oligopeptidase
3.4.21.27 coagulation factor XIa



benzyloxycarbonyl-Gly-Pro-p-nitroanilide + H2O <=> Benzyloxycarbonyl-Gly-Pro + 4-nitroaniline 3.4.21.26 prolyl oligopeptidase
3.4.21.27 coagulation factor XIa


N-acetyl-D-Phe-Gly_4-nitroanilide + H2O <=> N-acetyl-D-Phe-Gly + 4-nitroaniline 3.4.22.3 ficain

N-Acetyl-L-Phe-Gly_4-nitroanilide + H2O <=> N-acetyl-L-Phe-Gly + 4-nitroaniline 3.4.22.2 papain
3.4.22.3 ficain


Nalpha-benzoyl-Leu_4-nitroanilide + H2O <=> Nalpha-benzoyl-L-Leu + 4-nitroaniline 3.4.21.57 leucyl endopeptidase

N-acetyl-L-alanyl_4-nitroanilide + H2O <=> N-acetyl-L-alanine + 4-nitroaniline 3.4.11.B4
3.4.19.1 acylaminoacyl-peptidase
3.4.22.16 cathepsin H



Leu-Pro-p-nitroanilide + H2O <=> Leu-Pro + 4-nitroaniline 3.4.13.18 cytosol nonspecific dipeptidase
3.4.14.5 dipeptidyl-peptidase IV


5-L-glutamyl-4-nitroanilide + L-lysine <=> 4-nitroaniline + 5-L-glutamyl-L-lysine 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-proline <=> 4-nitroaniline + 5-L-glutamyl-L-proline 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-tyrosine <=> 4-nitroaniline + 5-L-glutamyl-L-tyrosine 2.3.2.2 gamma-glutamyltransferase

benzoyl-Asn-4-nitroanilide + H2O <=> N-benzoyl-L-asparagine + 4-nitroaniline 3.4.22.34 legumain
3.4.22.B1


benzyloxycarbonyl-Phe-Leu-4-nitroanilide + H2O <=> Benzyloxycarbonyl-Phe-Leu + 4-nitroaniline 3.4.22.2 papain

L-Thr-4-nitroanilide + H2O <=> L-threonine + 4-nitroaniline 3.4.11.1 leucyl aminopeptidase

acetyl-VDVAD-4-nitroanilide + H2O <=> acetyl-VDVAD + 4-nitroaniline 3.4.22.55 caspase-2

acetyl-L-Val-L-Asp-L-Val-L-Ala-L-Asp-4-nitroanilide + H2O <=> 4-nitroaniline + acetyl-VDVAD 3.4.22.55 caspase-2
3.4.22.56 caspase-3
3.4.22.60 caspase-7
3.4.22.61 caspase-8




acetyl-VEID-4-nitroanilide + H2O <=> acetyl-VEID + 4-nitroaniline 3.4.21.79 granzyme B
3.4.22.36 caspase-1
3.4.22.56 caspase-3
3.4.22.57 caspase-4
3.4.22.59 caspase-6
3.4.22.60 caspase-7






acetyl-Val-Glu-Ile-Asp-p-nitroanilide + H2O <=> acetyl-VEID + 4-nitroaniline 3.4.22.59 caspase-6
3.4.22.61 caspase-8


acetyl-YVAD-4-nitroanilide + H2O <=> acetyl-YVAD + 4-nitroaniline 3.4.21.79 granzyme B
3.4.22.36 caspase-1
3.4.22.56 caspase-3
3.4.22.57 caspase-4




(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + H2O <=> acetyl-YVAD + 4-nitroaniline 3.4.22.57 caspase-4

butyloxycarbonyl-L-Gln-L-Ala-L-Arg-4-nitroanilide + H2O <=> butyloxycarbonyl-Gln-Ala-Arg + 4-nitroaniline 3.4.21.109 matriptase

IETD-4-nitroanilide + H2O <=> IETD + 4-nitroaniline 3.4.22.56 caspase-3
3.4.22.61 caspase-8


succinyl-YVAD-4-nitroanilide + H2O <=> succinyl-YVAD + 4-nitroaniline 3.4.22.36 caspase-1
3.4.22.57 caspase-4


Asn-Pro-p-nitroanilide + H2O <=> Asn-Pro + 4-nitroaniline 3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV


N-methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide + H2O <=> methylsulfonyl-D-Phe-Gly-Arg + 4-nitroaniline 3.4.21.21 coagulation factor VIIa

methylsulfonyl-D-Phe-Gly-Arg-p-nitroanilide + H2O <=> methylsulfonyl-D-Phe-Gly-Arg + 4-nitroaniline 3.4.21.21 coagulation factor VIIa
3.4.21.68 t-plasminogen activator


Phe-Gly-Arg-p-nitroanilide + H2O <=> Phe-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

Ac-IEPD-p-nitroanilide + H2O <=> Ac-IEPD + 4-nitroaniline 3.4.21.79 granzyme B

acetyl-IEPD-p-nitroanilide + H2O <=> Ac-IEPD + 4-nitroaniline 3.4.21.79 granzyme B

S-benzyl-L-cysteine-p-nitroanilide + H2O <=> S-Benzyl-L-cysteine + 4-nitroaniline 3.4.11.3 cystinyl aminopeptidase

L-pyroGlu-L-Pro-L-Arg-4-nitroanilide + H2O <=> L-pyroglutamyl-L-prolyl-L-arginine + 4-nitroaniline 3.4.21.21 coagulation factor VIIa
3.4.21.27 coagulation factor XIa
3.4.21.68 t-plasminogen activator
3.4.21.69 protein C (activated)
3.4.21.73 u-plasminogen activator
3.4.21.B1






L-pyroglutamyl-L-Pro-L-Arg-4-nitroanilide + H2O <=> L-pyroglutamyl-L-prolyl-L-arginine + 4-nitroaniline 3.4.21.27 coagulation factor XIa

N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O <=> 4-nitroaniline + N-succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 3.4.21.1 chymotrypsin
3.4.21.39 chymase
3.4.21.B21



succinyl-Gly-Gly-p-nitroanilide + H2O <=> succinyl-Gly-Gly + 4-nitroaniline 3.4.21.1 chymotrypsin

Gly-L-Ala-L-Pro-4-nitroanilide + H2O <=> Gly-Ala-Pro + 4-nitroaniline 3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase

Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide + H2O <=> TSAVLQ + 4-nitroaniline 3.4.22.69 SARS coronavirus main proteinase

Boc-Leu-Ser-Thr-Arg-p-nitroanilide + H2O <=> 4-nitroaniline + Boc-Leu-Ser-Thr-Arg 3.4.21.69 protein C (activated)
3.4.21.74 venombin A


Z-Ala-Ala-Leu-p-nitroanilide + H2O <=> N-benzyloxycarbonyl-Ala-Ala-Leu + 4-nitroaniline 3.4.21.1 chymotrypsin
3.4.21.62 subtilisin
3.4.21.B52



ZAAL-4-nitroanilide + H2O <=> N-benzyloxycarbonyl-Ala-Ala-Leu + 4-nitroaniline 3.4.24.B15

Z-Ala-Ala-Leu-4-nitroanilide + H2O <=> N-benzyloxycarbonyl-Ala-Ala-Leu + 4-nitroaniline 3.4.24.11 neprilysin
3.4.24.B15


arachidonoyl_p-nitroanilide + H2O <=> arachidonic_acid + 4-nitroaniline 3.5.1.99 fatty acid amide hydrolase

Ser-Pro-p-nitroanilide + H2O <=> Ser-Pro + 4-nitroaniline 3.4.13.18 cytosol nonspecific dipeptidase
3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase
3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV




butyloxycarbonyl-L-Gln-L-Ala-L-Arg-4-nitroanilide + H2O <=> t-butyloxycarbonyl-Gln-Ala-Arg + 4-nitroaniline 3.4.21.109 matriptase

L-isoleucine_4-nitroanilide + H2O <=> L-isoleucine + 4-nitroaniline 3.4.11.1 leucyl aminopeptidase

L-isoleucyl_4-nitroanilide + H2O <=> L-isoleucine + 4-nitroaniline 3.4.11.B9

Ile-4-nitroanilide + H2O <=> L-isoleucine + 4-nitroaniline 3.4.11.1 leucyl aminopeptidase
3.4.11.22 aminopeptidase I


benzyloxycarbonyl-Arg_4-nitroanilide + H2O <=> Benzyloxycarbonyl-Arg + 4-nitroaniline 3.4.21.4 trypsin
3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.59 tryptase
3.4.21.B4
3.4.22.7 asclepain





benzyloxycarbonyl-Arg-p-nitroanilide + H2O <=> Benzyloxycarbonyl-Arg + 4-nitroaniline 3.4.21.35 tissue kallikrein

Nalpha-benzyloxycarbonyl-L-Phe-L-Arg-4-nitroanilide + H2O <=> Benzyloxycarbonyl-Phe-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.83 oligopeptidase B
3.4.22.15 cathepsin L
3.4.22.2 papain




N-benzyloxycarbonyl-Phe-Arg-4-nitroanilide + H2O <=> Benzyloxycarbonyl-Phe-Arg + 4-nitroaniline 3.4.22.27 cathepsin S

Phe-Pro-p-nitroanilide + H2O <=> Phe-Pro + 4-nitroaniline 3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase
3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV



Gly-Ala-4-nitroanilide + H2O <=> Gly-Ala + 4-nitroaniline 3.4.14.5 dipeptidyl-peptidase IV

Gly-Pro-Ala_4-nitroanilide + H2O <=> Gly-Pro-Ala + 4-nitroaniline 3.4.14.9 tripeptidyl-peptidase I

5-L-glutamyl-4-nitroanilide + L-aspartate <=> 4-nitroaniline + 5-L-Glutamyl-L-aspartate 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-methionine <=> 4-nitroaniline + 5-L-Glutamyl-L-methionine 2.3.2.2 gamma-glutamyltransferase

Benzyloxycarbonyl-citrulline_4-nitroanilide + H2O <=> Benzyloxycarbonyl-citrulline + 4-nitroaniline 3.4.22.32 stem bromelain

benzyloxycarbonyl-citrulline-p-nitroanilide + H2O <=> Benzyloxycarbonyl-citrulline + 4-nitroaniline 3.4.22.3 ficain

benzyloxycarbonyl-Phe-Leu-Glu-p-nitroanilide + H2O <=> Benzyloxycarbonyl-Phe-Leu-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

N-benzyloxycarbonyl-Phe-Leu-Glu-NH-p-nitroanilide + H2O <=> Benzyloxycarbonyl-Phe-Leu-Glu + 4-nitroaniline 3.4.22.48 staphopain

Benzyloxycarbonyl-Phe-Leu-Glu_2-nitroanilide + H2O <=> Benzyloxycarbonyl-Phe-Leu-Glu + 4-nitroaniline 3.4.21.82 glutamyl endopeptidase II

Z-Phe-Val-Arg-p-nitroanilide + H2O <=> Benzyloxycarbonyl-Phe-Val-Arg + 4-nitroaniline 3.4.21.B12

H-D-Cyclohexylglycyl-L-2-aminobutyryl-Arg_4-nitroanilide + H2O <=> H-D-Cyclohexylglycyl-L-alpha-aminobutyryl-L-arginine + 4-nitroaniline 3.4.21.21 coagulation factor VIIa
3.4.21.55 venombin AB
3.4.21.74 venombin A



His-p-nitroanilide + H2O <=> L-histidine + 4-nitroaniline 3.4.11.5 prolyl aminopeptidase
3.4.21.26 prolyl oligopeptidase


tert-butyloxycarbonyl-Ala-Ala-Pro-Glu_4-nitroanilide + H2O <=> tert-butyloxycarbonyl-Ala-Ala-Pro-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase
3.4.21.82 glutamyl endopeptidase II


Val-Leu-Arg-4-nitroanilide + H2O <=> Val-Leu-Arg + 4-nitroaniline 3.4.21.35 tissue kallikrein

Lys-Ala-p-nitroanilide + H2O <=> Lys-Ala + 4-nitroaniline 3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV


S-benzyl-L-cysteine-p-nitroanilide + H2O <=> S-benzyl-L-Cys + 4-nitroaniline 3.4.13.23 cysteinylglycine-S-conjugate dipeptidase

S-Benzoyl-Cys_4-nitroanilide + H2O <=> S-benzoyl-L-Cys + 4-nitroaniline 3.4.11.22 aminopeptidase I

Arg-Pro-Tyr_4-nitroanilide + H2O <=> Arg-Pro-Tyr + 4-nitroaniline 3.4.21.77 semenogelase

benzyloxycarbonyl-Ala-Glu-p-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

DL-Val-Leu-Arg-paranitroanilide + H2O <=> DL-Val-Leu-Arg + 4-nitroaniline 3.4.21.83 oligopeptidase B

DL-Val-Leu-Arg-p-nitroanilide + H2O <=> DL-Val-Leu-Arg + 4-nitroaniline 3.4.21.35 tissue kallikrein

Abz-Ala-Ala-Phe-4-nitroanilide + H2O <=> o-aminobenzoyl-Ala-Ala-Phe + 4-nitroaniline 3.4.21.12 alpha-lytic endopeptidase

N-succinyl-Ala-Pro-Ala-4-nitroanilide + H2O <=> succinyl-Ala-Pro-Ala + 4-nitroaniline 3.4.22.16 cathepsin H

Val-Leu-Lys-p-nitroanilide + H2O <=> Val-Leu-Lys + 4-nitroaniline 3.4.21.50 lysyl endopeptidase
3.4.21.89 signal peptidase I
3.4.22.B69



S-2251 + H2O <=> Val-Leu-Lys + 4-nitroaniline 3.4.22.47 gingipain K

benzyloxycarbonyl-Gly-4-nitroanilide + H2O <=> Benzyloxycarbonyl-Gly + 4-nitroaniline 3.4.21.62 subtilisin

oleoyl_p-nitroanilide + H2O <=> oleic_acid + 4-nitroaniline 3.5.1.99 fatty acid amide hydrolase

5-L-glutamyl-4-nitroanilide + L-isoleucine <=> 4-nitroaniline + 5-L-glutamyl-L-isoleucine 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-threonine <=> 4-nitroaniline + 5-L-glutamyl-L-threonine 2.3.2.2 gamma-glutamyltransferase

AcR-R-R-R-V-L-R-4-nitroanilide + H2O <=> AcR-R-R-R-V-L-R + 4-nitroaniline 3.4.24.83 anthrax lethal factor endopeptidase

succinyl-Ala-Ala-Pro-4-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro + 4-nitroaniline 3.4.21.36 pancreatic elastase
3.4.22.16 cathepsin H


formylmethionine_p-nitroanilide + H2O <=> N-formyl-L-methionine + 4-nitroaniline 3.4.19.1 acylaminoacyl-peptidase

nonanoyl_p-nitroanilide + H2O <=> Nonanoate + 4-nitroaniline 3.5.1.99 fatty acid amide hydrolase

acetyl-Trp-Glu-His-Asp-p-nitroanilide + H2O <=> acetyl-WEHD + 4-nitroaniline 3.4.22.61 caspase-8

Ala-Ala-Pro-4-nitroanilide + H2O <=> Ala-Ala-Pro + 4-nitroaniline 3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase
3.4.14.9 tripeptidyl-peptidase I
3.4.21.26 prolyl oligopeptidase



Ala-Ala-Pro-p-nitroanilide + H2O <=> Ala-Ala-Pro + 4-nitroaniline 3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase

methoxysuccinyl-Ala-Ala-Pro-Met-4-nitroanilide + H2O <=> methoxy-succinyl-Ala-Ala-Pro-Met + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme
3.4.21.39 chymase


methoxysuccinyl-Arg-Pro-Tyr-4-nitroanilide + H2O <=> methoxy-succinyl-Arg-Pro-Tyr + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme
3.4.21.39 chymase
3.4.21.77 semenogelase
3.4.21.B45
3.4.21.B55





N-succinyl-Ala-Pro-Ala-4-nitroanilide + H2O <=> N-succinyl-Ala-Pro-Ala + 4-nitroaniline 3.4.21.111 aqualysin 1

Nle-Nle-p-nitroanilide + H2O <=> Nle-Nle-OH + 4-nitroaniline 3.4.14.2 dipeptidyl-peptidase II

S2444 + H2O <=> Pyr-Gly-Arg + 4-nitroaniline 3.4.21.21 coagulation factor VIIa
3.4.21.59 tryptase
3.4.21.73 u-plasminogen activator
3.4.21.B1
3.4.24.72 fibrolase





N-succinyl-L-Val-L-Pro-L-Phe-4-nitroanilide + H2O <=> succinyl-Val-Pro-Phe + 4-nitroaniline 3.4.21.20 cathepsin G
3.4.21.B3


succinyl-Val-Pro-Phe-4-nitroanilide + H2O <=> succinyl-Val-Pro-Phe + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme

4-nitrophenyl_acetate + 4-nitroaniline <=> 4-nitrophenol + p-nitroacetanilide 2.3.1.5 arylamine N-acetyltransferase

L-benzyl-L-cysteine-p-nitroanilide + H2O <=> (S)-N-benzylcysteine + 4-nitroaniline 3.4.11.3 cystinyl aminopeptidase

Ala-Phe-4-nitroanailide + H2O <=> Ala-Phe + 4-nitroaniline 3.4.14.2 dipeptidyl-peptidase II

Arg-Pro-p-nitroanilide + H2O <=> Arg-Pro + 4-nitroaniline 3.4.13.18 cytosol nonspecific dipeptidase
3.4.14.11 Xaa-Pro dipeptidyl-peptidase
3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase
3.4.14.2 dipeptidyl-peptidase II
3.4.21.26 prolyl oligopeptidase





gamma-glutamyl-4-nitroanilide + hyroxamate <=> 4-nitroaniline + gamma-glutamyl_hydroxamate 2.3.2.2 gamma-glutamyltransferase

N-acetyl-Gly-p-nitroanilide + H2O <=> N-acetylglycine + 4-nitroaniline 3.4.19.1 acylaminoacyl-peptidase

Arg-Gly-Arg-4-nitroanilide + H2O <=> Arg-Gly-Arg + 4-nitroaniline 3.4.21.6 coagulation factor Xa

Val-Pro-4-nitroanilide + H2O <=> Val-Pro + 4-nitroaniline 3.4.14.5 dipeptidyl-peptidase IV

N-succinyl-Ala-Phe-p-nitroanilide + H2O <=> N-succinyl-Ala-Phe + 4-nitroaniline 3.4.21.1 chymotrypsin

N-succinyl-Ala-Pro-4-nitroanilide + H2O <=> N-succinyl-Ala-Phe + 4-nitroaniline 3.4.21.26 prolyl oligopeptidase

N-succinyl-Ala-Ala-Pro-Met-p-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Met + 4-nitroaniline 3.4.21.1 chymotrypsin
3.4.21.62 subtilisin
3.4.21.71 pancreatic elastase II



N-succinyl-Ala-Ala-Pro-Lys-p-nitroanilide + H2O <=> N-succinyl-Ala-Ala-Pro-Lys + 4-nitroaniline 3.4.21.1 chymotrypsin
3.4.21.62 subtilisin
3.4.21.81 streptogrisin B
3.4.21.83 oligopeptidase B
3.4.21.B55





D-cyclohexylglycyl-Gly-Arg-p-nitroanilide + H2O <=> D-cyclohexylglycyl-Gly-Arg + 4-nitroaniline 3.4.21.22 coagulation factor IXa
3.4.21.68 t-plasminogen activator


succinyl-Gly-L-Pro-4-nitroanilide + H2O <=> succinyl-Gly-Pro + 4-nitroaniline 3.4.21.26 prolyl oligopeptidase

succinyl-AAPR-p-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Arg + 4-nitroaniline 3.4.21.1 chymotrypsin
3.4.21.5 thrombin
3.4.21.83 oligopeptidase B
3.4.21.B55




benzoyl-Ile-Glu(gamma-O-methyl)-Gly-Arg-4-nitroanilide + S2222 + H2O <=> benzoyl-Ile-Glu(gamma-O-methyl)-Gly-Arg + N-benzoyl-Ile-Glu-Gly-Arg + 4-nitroaniline 3.4.21.6 coagulation factor Xa

S2222 + H2O <=> N-benzoyl-Ile-Glu-Gly-Arg + 4-nitroaniline 3.4.21.4 trypsin
3.4.21.6 coagulation factor Xa
3.4.21.60 scutelarin
3.4.21.74 venombin A




benzoyl-Ile-Glu-Gly-Arg_p-nitroanilide + H2O <=> N-benzoyl-Ile-Glu-Gly-Arg + 4-nitroaniline 3.4.21.48 cerevisin

benzyloxycarbonyl-D-Glu-Gly-Arg-p-nitroanilide + H2O <=> benzyloxycarbonyl-D-Glu-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

benzyloxycarbonyl-D-Glu(O-tert-butyl)-Gly-Arg-p-nitroanilide + H2O <=> benzyloxycarbonyl-D-Glu-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

methoxycarbonyl-D-Nle-Gly-Arg-p-nitroanilide + H2O <=> methyloxycarbonyl-D-Nle-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

methyloxycarbonyl-D-Val-Gly-Arg-p-nitroanilide + H2O <=> methyloxycarbonyl-D-Nle-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

methyloxycarbonyl-D-cyclohexylalanyl-Gly-Arg-p-nitroanilide + H2O <=> methyloxycarbonyl-D-cyclohexylglycyl-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

methyloxycarbonyl-D-cyclohexylglycyl-Gly-Arg-p-nitroanilide + H2O <=> methyloxycarbonyl-D-cyclohexylglycyl-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

D-Glu-Gly-Arg-p-nitroanilide + H2O <=> D-Glu-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator
3.4.21.73 u-plasminogen activator


alpha-N-benzoyl-Val-Gly-Arg-4-nitroanilide + H2O <=> benzoyl-Val-Gly-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.B3
3.4.22.37 gingipain R



5-L-glutamyl-4-nitroanilide + L-histidine <=> 4-nitroaniline + 5-L-glutamyl-L-His 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-tryptophan <=> 4-nitroaniline + 5-L-glutamyl-L-Trp 2.3.2.2 gamma-glutamyltransferase

N-glutaryl-L-Phe-p-nitroanilide + H2O <=> N-glutaryl-L-phenylalanine + 4-nitroaniline 3.4.21.1 chymotrypsin

L-Arg-L-Arg-4-nitroanilide + H2O <=> Arg-Arg + 4-nitroaniline 3.4.22.1 cathepsin B

benzyloxycarbonyl-L-Arg-L-Arg-4-nitroanilide + H2O <=> Arg-Arg + 4-nitroaniline 3.4.22.1 cathepsin B

benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O <=> benzyloxycarbonyl-Lys + 4-nitroaniline + H2O 3.4.21.42 complement subcomponent C_overbar_1s_
3.4.22.47 gingipain K


Cbz-L-Lys-4-nitroanilide + H2O <=> benzyloxycarbonyl-Lys + 4-nitroaniline 3.4.21.B4

Phe-Ala-4-nitroanilide + H2O <=> Phe-Ala + 4-nitroaniline 3.4.14.5 dipeptidyl-peptidase IV

Suc-Phe-4-nitroanilide + H2O <=> 4-nitroaniline + N-succinyl-Phe 3.4.21.1 chymotrypsin

L-pyroGlu-L-Pro-L-Arg-4-nitroanilide + H2O <=> 4-nitroaniline + pyroGlu-Pro-Arg 3.4.21.21 coagulation factor VIIa
3.4.21.69 protein C (activated)


Tyr-Pro-4-nitroanilide + H2O <=> Tyr-Pro + 4-nitroaniline 3.4.14.5 dipeptidyl-peptidase IV

beta-Ala-p-nitroanilide + H2O <=> beta-Alanine + 4-nitroaniline 3.4.11.25 beta-peptidyl aminopeptidase
3.4.13.20 beta-Ala-His dipeptidase


5-L-glutamyl-4-nitroanilide + Gly-Gly-Ala <=> 4-nitroaniline + 5-L-glutamyl-Gly-Gly-Ala 2.3.2.2 gamma-glutamyltransferase

palmitoyl_p-nitroanilide + H2O <=> palmitate + 4-nitroaniline 3.5.1.99 fatty acid amide hydrolase

Pro-Ala-p-nitroanilide + H2O <=> Pro-Ala + 4-nitroaniline 3.4.14.2 dipeptidyl-peptidase II

Pro-Ala-4-nitroanilide + H2O <=> Pro-Ala + 4-nitroaniline 3.4.14.5 dipeptidyl-peptidase IV

acetyl-Asp-p-nitroanilide + H2O <=> N-acetyl-L-glutamate + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

N-acetyl-Glu_p-nitroanilide + H2O <=> N-acetyl-L-glutamate + 4-nitroaniline 3.4.19.1 acylaminoacyl-peptidase
3.4.21.19 glutamyl endopeptidase


5-[(4-nitrophenyl)amino]-5-oxopentanoic_acid + H2O <=> Glutarate + 4-nitroaniline 3.5.1.4 amidase

Z-Ala-Leu-Arg-nitroanilide + H2O <=> 4-nitroaniline + Z-Ala-Leu-Arg 3.4.21.9 enteropeptidase

D-Phe-Pro-Arg-4-nitroanilide + H2O <=> D-FPR + 4-nitroaniline 3.4.21.5 thrombin

D-Phe-L-Pro-L-Arg-4-nitroanilide + H2O <=> D-FPR + 4-nitroaniline 3.4.21.5 thrombin

Bz-Phe-Val-Arg-4-nitroanilide + H2O <=> Benzoyl-Phe-Val-Arg + 4-nitroaniline 3.4.21.34 plasma kallikrein
3.4.21.4 trypsin
3.4.21.59 tryptase
3.4.21.74 venombin A
3.4.22.32 stem bromelain
3.4.22.37 gingipain R
3.4.22.B1
3.4.22.B50








benzoyl-Phe-Val-Arg-p-nitroanilide + H2O <=> Benzoyl-Phe-Val-Arg + 4-nitroaniline 3.4.22.1 cathepsin B
3.4.22.33 fruit bromelain


His-Pro-p-nitroanilide + H2O <=> His-Pro + 4-nitroaniline 3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV


Lys-Pro-p-nitroanilide + H2O <=> Lys-Pro + 4-nitroaniline 3.4.13.18 cytosol nonspecific dipeptidase
3.4.14.11 Xaa-Pro dipeptidyl-peptidase
3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase
3.4.14.2 dipeptidyl-peptidase II




Gly-Pro-4-nitroanilide + H2O <=> Lys-Pro + 4-nitroaniline 3.4.14.5 dipeptidyl-peptidase IV

L-Pro-Phe-Arg-p-nitroanilide + H2O <=> Pro-Phe-Arg + 4-nitroaniline 3.4.21.B45

Ile-Pro-4-nitroanilide + H2O <=> Ile-Pro + 4-nitroaniline 3.4.14.5 dipeptidyl-peptidase IV

5-L-glutamyl-4-nitroanilide + acceptor <=> 4-nitroaniline + 5-L-Glutamyl-acceptor 2.3.2.2 gamma-glutamyltransferase

L-Ala-L-Ala-L-Phe_4-nitroanilide + H2O <=> Ala-Ala-Phe + 4-nitroaniline 3.4.14.10 tripeptidyl-peptidase II

Ala-Ala-Phe-4-nitroanilide + H2O <=> Ala-Ala-Phe + 4-nitroaniline 3.4.11.B9
3.4.14.10 tripeptidyl-peptidase II
3.4.14.9 tripeptidyl-peptidase I



Ala-Ala-Phe-p-nitroanilide + H2O <=> Ala-Ala-Phe + 4-nitroaniline 3.4.11.4 tripeptide aminopeptidase
3.4.14.10 tripeptidyl-peptidase II
3.4.21.100 sedolisin



benzyloxycarbonyl-Glu-p-nitroanilide + H2O <=> Benzyloxycarbonyl-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

Ac-Leu-p-nitroanilide + H2O <=> N-acetyl-L-leucine + 4-nitroaniline 3.4.11.B4
3.4.11.B5
3.4.11.B6
3.4.19.1 acylaminoacyl-peptidase




succinyl-Ala-Ala-p-nitroanilide + H2O <=> Succinyl-Ala-Ala + 4-nitroaniline 3.4.21.62 subtilisin

Suc-Ala-Ala-p-nitroanilide + H2O <=> Succinyl-Ala-Ala + 4-nitroaniline 3.4.22.16 cathepsin H

Ac-Arg-Val-Arg-Arg-4-nitroanilide + H2O <=> acetyl-RVRR + 4-nitroaniline 3.4.21.75 furin

5-D-glutamyl-4-nitroanilide + acceptor <=> 4-nitroaniline + 5-D-glutamyl-acceptor 2.3.2.2 gamma-glutamyltransferase

benzoyl-DL-Arg-p-nitroanilide + H2O <=> benzoyl-DL-Arg + 4-nitroaniline 3.4.21.4 trypsin
3.4.22.1 cathepsin B
3.4.22.3 ficain



D-Val-Leu-Arg-p-nitroanilide + H2O <=> D-Val-Leu-Arg + 4-nitroaniline 3.4.21.118 kallikrein 8
3.4.21.34 plasma kallikrein
3.4.21.35 tissue kallikrein
3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.B12
3.5.1.4 amidase






N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O <=> MeO-succinyl-Ala-Ala-Pro-Val + 4-nitroaniline 3.4.21.39 chymase

N-methoxycarbonyl-D-Nle-Gly-Arg-p-nitroanilide + H2O <=> N-methoxycarbonyl-D-Nle-Gly-Arg + 4-nitroaniline 3.4.21.21 coagulation factor VIIa

succinyl-Ala-His-(cis)-Pro-Phe_4-nitroanilide + H2O <=> N-succinyl-Ala-His-Pro-Phe + 4-nitroaniline 3.4.21.39 chymase

5-L-glutamyl-4-nitroanilide + L-lysine <=> 4-nitroaniline + 2-N-(5-L-Glutamyl)L-lysine 2.3.2.2 gamma-glutamyltransferase

benzoyl-Arg-4-nitroanilide + H2O <=> N-benzoyl-Arg + 4-nitroaniline 3.4.21.6 coagulation factor Xa
3.4.22.6 chymopapain


Nalpha-benzoyl-Val_4-nitroanilide + H2O <=> N-benzoyl-L-valine + 4-nitroaniline 3.4.21.57 leucyl endopeptidase

5-L-glutamyl-4-nitroanilide + 5-L-glutamyl-4-nitroanilide <=> 5-L-Glutamyl-5-L-glutamyl-4-nitroanilide + 4-nitroaniline 2.3.2.2 gamma-glutamyltransferase

Benzoyl-L-Lys-Gly-L-Arg_4-nitroanilide + H2O <=> Benzoyl-L-Lys-Gly-L-Arg + 4-nitroaniline 3.4.21.59 tryptase

benzyloxycarbonyl-Glu-Phe-Arg_4-nitroanilide + H2O <=> Benzyloxycarbonyl-Glu-Phe-Arg + 4-nitroaniline 3.4.21.59 tryptase

5-L-glutamyl-4-nitroanilide + L-serine <=> 4-nitroaniline + gamma-L-Glu-L-Ser 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-threonine <=> 4-nitroaniline + gamma-L-Glu-L-Thr 2.3.2.2 gamma-glutamyltransferase

H-D-Aminobutyric_acid-cyclohexylalanyl-Lys_4-nitroanilide + H2O <=> H-D-Aminobutyric_acid-cyclohexylalanyl-Lys + 4-nitroaniline 3.4.21.74 venombin A

H-D-Cyclohexylglycyl-aminobutyric_acid-cyclohexylalanyl-Lys_4-nitroanilide + H2O <=> H-D-Cyclohexylglycyl-aminobutyric_acid-cyclohexylalanyl-Lys + 4-nitroaniline 3.4.21.74 venombin A

spectrozyme-TH + H2O <=> H-D-Hexahydrotyrosyl-Ala-Arg + 4-nitroaniline 3.4.21.6 coagulation factor Xa
3.4.21.74 venombin A


H-D-Nle-cyclohexylalanyl-Arg_4-nitroanilide + H2O <=> H-D-Nle-cyclohexylalanyl-L-arginine + 4-nitroaniline 3.4.21.74 venombin A

D-norleucyl-hexahydrotyrosyl-lysine-p-nitroanilide + H2O <=> H-D-Nle-hexahydrotyrosyl-Lys + 4-nitroaniline 3.4.21.68 t-plasminogen activator
3.4.21.7 plasmin
3.4.21.74 venombin A



H-D-Phenylglycine-Phe-Arg_4-nitroanilide + H2O <=> H-D-Phenylglycyl-Phe-Arg + 4-nitroaniline 3.4.21.74 venombin A

H-D-Pro-hexahydrotyrosyl-Arg_4-nitroanilide + H2O <=> H-D-Pro-hexahydrotyrosyl-L-arginine + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.74 venombin A


L-Phe-pipecolyl-L-Arg_4-nitroanilide + H2O <=> L-Phe-pipecolyl-L-Arg + 4-nitroaniline 3.4.21.95 snake venom factor V activator

Methylsulfonyl-Leu-Gly-Arg_4-nitroanilide + H2O <=> Methylsulfonyl-Leu-Gly-Arg + 4-nitroaniline 3.4.21.74 venombin A

Tert-Butoxycarbonyl-Ala-Ala-Pro-Asp_4-nitroanilide + H2O <=> Tert-Butoxycarbonyl-Ala-Ala-Pro-Asp + 4-nitroaniline 3.4.21.82 glutamyl endopeptidase II

Tert-Butyloxycarbonyl-Ala-Phe-Pro-Glu_4-nitroanilide + H2O <=> Tert-Butoxycarbonyl-Ala-Phe-Pro-Glu + 4-nitroaniline 3.4.21.82 glutamyl endopeptidase II

tert-butyloxycarbonyl-Ala-Ala-Ala-Glu_4-nitroanilide + H2O <=> tert-butyloxycarbonyl-Ala-Ala-Ala-Glu + 4-nitroaniline 3.4.21.82 glutamyl endopeptidase II

tert-butyloxycarbonyl-Ala-Ala-Gly-Glu_4-nitroanilide + H2O <=> tert-butyloxycarbonyl-Ala-Ala-Gly-Glu + 4-nitroaniline 3.4.21.82 glutamyl endopeptidase II

Tert-Butyloxycarbonyl-Ala-Ala-Phe-Glu_4-nitroanilide + H2O <=> tert-butyloxycarbonyl-Ala-Ala-Phe-Glu + 4-nitroaniline 3.4.21.82 glutamyl endopeptidase II

Tert-Butyloxycarbonyl-Ala-Leu-Pro-Glu_4-nitroanilide + H2O <=> Tert-Butyloxycarbonyl-Ala-Leu-Pro-Glu + 4-nitroaniline 3.4.21.82 glutamyl endopeptidase II

Tert-Butyloxycarbonyl-Ala-Pro-Glu_4-nitroanilide + H2O <=> Tert-Butyloxycarbonyl-Ala-Pro-Glu + 4-nitroaniline 3.4.21.82 glutamyl endopeptidase II

Tosyl-citrulline_4-nitroanilide + H2O <=> Tosyl-citrulline + 4-nitroaniline 3.4.22.32 stem bromelain

Tripeptide_nitroanilide_derivatives + H2O <=> Tripeptide + 4-nitroaniline 3.4.21.55 venombin AB

Carbobenzoxyl-Glu-(alpha-t-butoxy)-Gly-Arg_4-nitroanilide + H2O <=> Carbobenzoxyl-Glu-(alpha-t-butoxy)-Gly-Arg + 4-nitroaniline 3.4.21.74 venombin A

acetyl-Ala-Thr-Arg-p-nitroanilide + H2O <=> acetyl-Ala-Thr-Arg + 4-nitroaniline 3.4.21.35 tissue kallikrein

acetyl-citrulline-p-nitroanilide + H2O <=> N2-acetyl-L-citrulline + 4-nitroaniline 3.4.22.3 ficain

acetyl-Glu-Leu-Arg-p-nitroanilide + H2O <=> acetyl-Glu-Leu-Arg + 4-nitroaniline 3.4.21.35 tissue kallikrein

acetyl-Glu-Phe-Arg-p-nitroanilide + H2O <=> acetyl-Glu-Phe-Arg + 4-nitroaniline 3.4.21.35 tissue kallikrein

acetyl-Glu-Thr-Arg-p-nitroanilide + H2O <=> acetyl-Glu-Thr-Arg + 4-nitroaniline 3.4.21.35 tissue kallikrein

L-Ala-L-Ala-L-Ala-L-Pro-4-nitroanilide + H2O <=> Ala-Ala-Ala-Pro + 4-nitroaniline 3.4.21.26 prolyl oligopeptidase

benzyloxycarbonyl-Ala-Leu-Glu-p-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Leu-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

benzyloxycarbonyl-Leu-Glu-p-nitroanilide + H2O <=> benzyloxycarbonyl-Leu-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

Bz-Pro-Phe-Arg-NHPhNO2 + H2O <=> benzyloxycarbonyl-Pro-Phe-Arg + 4-nitroaniline 3.4.21.9 enteropeptidase
3.4.21.B39


Gly-hydroxyproline-4-nitroanilide + H2O <=> Gly-hydroxyproline + 4-nitroaniline 3.4.14.5 dipeptidyl-peptidase IV

succinyl-Ala-p-nitroanilide + H2O <=> succinyl-Ala + 4-nitroaniline 3.4.21.25 cucumisin

succinyl-Phe-4-nitroanilide + H2O <=> succinyl-Phe + 4-nitroaniline 3.4.21.39 chymase

t-butyloxycarbonyl-Ala-Ala-Asp-p-nitroanilide + H2O <=> t-butyloxycarbonyl-Ala-Ala-Asp + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

t-butyloxycarbonyl-Ala-Ala-Glu-p-nitroanilide + H2O <=> t-butyloxycarbonyl-Ala-Ala-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

octanoyl_p-nitroanilide + H2O <=> Octanoate + 4-nitroaniline 3.5.1.99 fatty acid amide hydrolase

Glu-Asp-Val-Val-4-aminobutyryl-Cys-p-nitroanilide + H2O <=> Glu-Asp-Val-Val-4-aminobutyryl-Cys + 4-nitroaniline 3.4.21.98 hepacivirin

myristoyl_p-nitroanilide + H2O <=> myristic_acid + 4-nitroaniline 3.5.1.99 fatty acid amide hydrolase

acetyl-Phe-Arg-p-nitroanilide + H2O <=> Acetyl-Phe-Arg + 4-nitroaniline 3.4.21.34 plasma kallikrein
3.4.21.35 tissue kallikrein


acetyl-Phe-Arg-4-nitroanilide + H2O <=> Acetyl-Phe-Arg + 4-nitroaniline 3.4.21.34 plasma kallikrein

lauroyl_p-nitroanilide + H2O <=> laurate + 4-nitroaniline 3.5.1.99 fatty acid amide hydrolase

N-benzoyl-L-Lys_p-nitroanilide + H2O <=> Benzoyl-Lys + 4-nitroaniline 3.4.21.50 lysyl endopeptidase

benzoyl-Lys-p-nitroanilide + H2O <=> Benzoyl-Lys + 4-nitroaniline 3.4.21.50 lysyl endopeptidase

N-alpha-benzoyl-L-Lys-4-nitroanilide + H2O <=> Benzoyl-Lys + 4-nitroaniline 3.4.21.4 trypsin
3.4.21.50 lysyl endopeptidase
3.4.21.57 leucyl endopeptidase
3.4.22.8 clostripain




benzyloxycarbonyl-Tyr-4-nitroanilide + H2O <=> Benzyloxycarbonyl-Tyr + 4-nitroaniline 3.4.21.B52

D-Pro-Phe-Arg-p-nitroanilide + H2O <=> D-Pro-Phe-Arg + 4-nitroaniline 3.4.21.21 coagulation factor VIIa
3.4.21.34 plasma kallikrein
3.4.21.35 tissue kallikrein
3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.74 venombin A
3.4.21.78 granzyme A
3.4.21.B12
3.4.22.1 cathepsin B
3.4.24.B39









5-L-glutamyl-4-nitroanilide + L-2-aminobutanoate <=> 4-nitroaniline + gamma-Glu-2-aminobutyrate 2.3.2.2 gamma-glutamyltransferase

S2444 + H2O <=> L-pyroGlu-Gly-L-Arg + 4-nitroaniline 3.4.21.73 u-plasminogen activator

5-L-glutamyl-4-nitroanilide + ethylamine <=> L-Theanine + 4-nitroaniline 2.3.2.2 gamma-glutamyltransferase

N-benzoyl-L-Tyr-p-nitroanilide + H2O <=> N-Benzoyl-L-tyrosine + 4-nitroaniline 3.4.21.1 chymotrypsin

benzoyl-Tyr-p-nitroanilide + H2O <=> N-Benzoyl-L-tyrosine + 4-nitroaniline 3.4.16.5 carboxypeptidase C
3.4.21.1 chymotrypsin
3.4.21.39 chymase
3.4.21.48 cerevisin




N-benzoyl-Tyr_p-nitroanilide + H2O <=> N-Benzoyl-L-tyrosine + 4-nitroaniline 3.4.16.5 carboxypeptidase C
3.4.21.1 chymotrypsin


5-L-glutamyl-4-nitroanilide + 1-aminocyclopropane-1-carboxylate <=> 4-nitroaniline + 5-L-glutamyl-1-aminocyclopropane-1-carboxylic_acid 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + citrulline <=> 4-nitroaniline + 5-L-glutamyl-citrulline 2.3.2.2 gamma-glutamyltransferase

L-Glu-4-nitroanilide + glutathione <=> 4-nitroaniline + 5-L-glutamyl-glutathione 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + Gly-Pro <=> 4-nitroaniline + 5-L-glutamyl-glycyl-L-proline 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + Ala-Gly <=> 4-nitroaniline + 5-L-glutamyl-L-alanylglycine 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-arginine <=> 4-nitroaniline + 5-L-glutamyl-L-arginine 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-asparagine <=> 4-nitroaniline + 5-L-glutamyl-L-asparagine 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-cystine <=> 4-nitroaniline + 5-L-glutamyl-L-cystine 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-histidine <=> 4-nitroaniline + 5-L-glutamyl-L-histidine 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-hydroxyproline <=> 4-nitroaniline + 5-L-glutamyl-L-hydroxyproline 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-leucyl-L-alanine <=> 4-nitroaniline + 5-L-glutamyl-L-leucyl-L-alanine 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-serine <=> 4-nitroaniline + 5-L-glutamyl-L-serine 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + S-alkylcysteine_sulfoxide <=> 4-nitroaniline + 5-L-glutamyl-S-alkylcysteine_sulfoxide 2.3.2.2 gamma-glutamyltransferase

tosyl-glycyl-prolyl-D-arginine-4-nitroanilide + H2O <=> Tosyl-Gly-Pro-Arg + 4-nitroaniline 3.4.21.6 coagulation factor Xa

tosyl-Gly-Pro-Arg-p-nitroanilide + H2O <=> Tosyl-Gly-Pro-Arg + 4-nitroaniline 3.4.21.35 tissue kallikrein
3.4.21.59 tryptase
3.4.21.B3
3.4.22.51 cruzipain




tosyl-Gly-Pro-Arg-4-nitroanilide + H2O <=> Tosyl-Gly-Pro-Arg + 4-nitroaniline 3.4.21.4 trypsin
3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.5 thrombin
3.4.21.59 tryptase
3.4.21.74 venombin A
3.4.21.83 oligopeptidase B
3.4.21.B45
3.4.22.26 cancer procoagulant
3.4.22.37 gingipain R









N-p-tosyl-Gly-Pro-Arg-p-nitroanilide + H2O <=> Tosyl-Gly-Pro-Arg + 4-nitroaniline 3.4.21.59 tryptase

Pro-Pro-p-nitroanilide + H2O <=> Pro-Pro + 4-nitroaniline 3.4.14.5 dipeptidyl-peptidase IV

D-Val-L-Leu-L-Lys-4-nitroanilide + H2O <=> D-Val-Leu-Lys + 4-nitroaniline 3.4.21.118 kallikrein 8
3.4.21.35 tissue kallikrein
3.4.21.7 plasmin
3.4.21.73 u-plasminogen activator
3.4.21.74 venombin A
3.4.21.B12
3.4.21.B55
3.4.24.72 fibrolase








D-Val-Leu-Lys-p-nitroanilide + H2O <=> D-Val-Leu-Lys + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_
3.4.21.62 subtilisin
3.4.21.68 t-plasminogen activator
3.4.21.7 plasmin
3.4.22.1 cathepsin B
3.4.22.47 gingipain K
3.4.23.48 plasminogen activator Pla







Gly-Arg_4-nitroanilide + H2O <=> Gly-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

Gly-Arg-p-nitroanilide + H2O <=> Gly-Arg + 4-nitroaniline 3.4.14.1 dipeptidyl-peptidase I

alpha-N-t-butoxycarbonyl-Gly-Phe-Arg-4-nitroanilide + H2O <=> N-t-butoxycarbonyl-Gly-Phe-Arg + 4-nitroaniline 3.4.21.B3

AcM-L-A-R-R-R-P-V-L-P-4-nitroanilide + H2O <=> AcM-L-A-R-R-R-P-V-L-P + 4-nitroaniline 3.4.24.83 anthrax lethal factor endopeptidase

D-Val-Phe-Lys-p-nitroanilide + H2O <=> D-Val-Phe-Lys + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_
3.4.22.47 gingipain K


N-2-benzyloxycarbonyl-D-arginyl-L-arginine-4-nitroanilide + H2O <=> N-2-benzyloxycarbonyl-D-arginyl-L-arginine + 4-nitroaniline 3.4.21.6 coagulation factor Xa

N-benzyloxycarbonyl-FR-4-nitroanilide + H2O <=> N-benzyloxycarbonyl-FR + 4-nitroaniline 3.4.22.B49

N-succinyl-Gly-Gly-4-nitroanilide + H2O <=> N-succinyl-Gly-Gly + 4-nitroaniline 3.4.21.1 chymotrypsin

benzyloxycarbonyl-L-Arg-L-Arg-4-nitroanilide + H2O <=> Nalpha-benzoyl-L-arginyl-L-arginine + 4-nitroaniline 3.4.21.83 oligopeptidase B

benzyloxycarbonyl-L-Lys-L-Arg_4-nitroanilide + H2O <=> Nalpha-benzoyl-L-lysyl-L-arginine + 4-nitroaniline 3.4.21.83 oligopeptidase B

Nalpha-benzyloxycarbonyl-L-arginine-4-nitroanilide + H2O <=> Nalpha-benzyloxycarbonyl-L-arginine + 4-nitroaniline 3.4.21.74 venombin A

Nalpha-CBZ_L-arginine-4-nitroanilide + H2O <=> Nalpha-CBZ_L-arginine + 4-nitroaniline 3.4.21.95 snake venom factor V activator

acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg-4-nitroanilide + H2O <=> acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg + 4-nitroaniline 3.4.21.4 trypsin

acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Arg-4-nitroanilide + H2O <=> acetyl-Ala-Val-(L-alpha-aminobutyryl)-Pro-Har + 4-nitroaniline 3.4.21.4 trypsin

acetyl-LEVD-4-nitroanilide + H2O <=> acetyl-LEVD + 4-nitroaniline 3.4.22.36 caspase-1
3.4.22.57 caspase-4
3.4.22.59 caspase-6



acetyl-YEID-4-nitroanilide + H2O <=> acetyl-YEID + 4-nitroaniline 3.4.22.60 caspase-7

acetyl-YQVD-4-nitroanilide + H2O <=> acetyl-YQVD + 4-nitroaniline 3.4.22.60 caspase-7

alpha-N-succinyl-Ser-Thr-Pro-Lys-4-nitroanilide + H2O <=> alpha-N-succinyl-Ser-Thr-Pro-Lys + 4-nitroaniline 3.4.21.B3

benzyloxycarbonyl-Ala-Ala-Glu-4-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Ala-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

benzyloxycarbonyl-Glu-Gly-Arg-4-nitroanilide + H2O <=> benzyloxycarbonyl-Glu-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

carbobenzoxyl-Glu-(alpha-tert-butoxy)-Gly-Arg_4-nitroanilide + H2O <=> carbobenzoxyl-Glu-(alpha-tert-butoxy)-Gly-Arg + 4-nitroaniline 3.4.21.74 venombin A

D-Asp(O-tert-butyl)-Gly-Arg-4-nitroanilide + H2O <=> D-Asp(O-tert-butyl)-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

D-butyl-cyclohexylalanine-arginine-4-nitroanilide + H2O <=> D-butyl-cyclohexylalanine-arginine + 4-nitroaniline 3.4.21.34 plasma kallikrein

L-Ile-Pro-Arg-4-nitroanilide + H2O <=> L-Ile-Pro-Arg + 4-nitroaniline 3.4.21.B39

N-carbobenzoxy-VVR-4-nitroanilide + H2O <=> N-carbobenzoxy-VVR + 4-nitroaniline 3.4.22.51 cruzipain

Nalpha-benzoyl-Ile-Glu(-OR)-Gly-Arg-4-nitroanilide + H2O <=> Nalpha-benzoyl-Ile-Glu(-OR)-Gly-Arg + 4-nitroaniline 3.4.21.38 coagulation factor XIIa

pGlu-Gly-Arg-4-nitroanilide + H2O <=> pGlu-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

succinyl-Met-Val-Pro-Phe-4-nitroanilide + H2O <=> succinyl-Met-Val-Pro-Phe + 4-nitroaniline 3.4.21.39 chymase

succinyl-Phe-Ser-Phe-4-nitroanilide + H2O <=> succinyl-Phe-Ser-Phe + 4-nitroaniline 3.4.21.39 chymase

succinyl-Phe-Val-Pro-Phe-4-nitroanilide + H2O <=> succinyl-Phe-Val-Pro-Phe + 4-nitroaniline 3.4.21.39 chymase

tert-butyloxycarbonyl-Ala-Ala-Leu-Glu_4-nitroanilide + H2O <=> tert-butyloxycarbonyl-Ala-Ala-Leu-Glu + 4-nitroaniline 3.4.21.82 glutamyl endopeptidase II

N-benzyloxycarbonyl-Phe-Leu-Glu-NH-p-nitroanilide + H2O <=> N-benzyloxycarbonyl-Phe-Leu-Glu + 4-nitroaniline 3.4.22.48 staphopain

N-alpha-acetyl-Arg-p-nitroanilide + H2O <=> acetyl-L-Arg + 4-nitroaniline 3.4.21.35 tissue kallikrein

succinyl-Ala-Ala-Ala-Ala-4-nitroanilide + H2O <=> succinyl-Ala-Ala-Ala-Ala + 4-nitroaniline 3.4.22.16 cathepsin H

succinyl-Ala-Ala-Ala-Ala-Ala-4-nitroanilide + H2O <=> succinyl-Ala-Ala-Ala-Ala-Ala + 4-nitroaniline 3.4.22.16 cathepsin H

succinyl-Pro-Ala-Ala-4-nitroanilide + H2O <=> succinyl-Pro-Ala-Ala + 4-nitroaniline 3.4.22.16 cathepsin H

5-L-glutamyl-4-nitroanilide + Glycine_methyl_ester <=> 4-nitroaniline + 5-L-glutamyl-glycyl_methylester 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + glycylglycine_methylester <=> 4-nitroaniline + 5-L-glutamyl-glycylglycine_methylester 2.3.2.2 gamma-glutamyltransferase

(5-L-glutamyl)-4-nitroanilide + Gly-Gly <=> 4-nitroaniline + gamma-glutamylglycylglycine 2.3.2.2 gamma-glutamyltransferase

acetyl-DQMD-4-nitroanilide + H2O <=> acetyl-DQMD + 4-nitroaniline 3.4.22.36 caspase-1
3.4.22.56 caspase-3
3.4.22.57 caspase-4
3.4.22.59 caspase-6
3.4.22.60 caspase-7





acetyl-Phe-Val-Arg-4-nitroanilide + H2O <=> acetyl-Phe-Val-Arg + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme

acetyl-VDVAD-4-nitroanilide + H2O <=> acetyl-VAVAD + 4-nitroaniline 3.4.22.56 caspase-3
3.4.22.60 caspase-7


acetyl-VDQQD-4-nitroanilide + H2O <=> acetyl-VDQQD + 4-nitroaniline 3.4.22.55 caspase-2
3.4.22.56 caspase-3
3.4.22.59 caspase-6
3.4.22.60 caspase-7




Ac-VDQQD-4-nitroanilide + H2O <=> acetyl-VDQQD + 4-nitroaniline 3.4.22.56 caspase-3

acetyl-VQVD-4-nitroanilide + H2O <=> acetyl-VQVD + 4-nitroaniline 3.4.22.36 caspase-1
3.4.22.56 caspase-3
3.4.22.57 caspase-4
3.4.22.59 caspase-6
3.4.22.60 caspase-7





acetyl-YEVD-4-nitroanilide + H2O <=> acetyl-YEVD + 4-nitroaniline 3.4.22.36 caspase-1
3.4.22.56 caspase-3
3.4.22.57 caspase-4
3.4.22.59 caspase-6
3.4.22.60 caspase-7





benzoyl-L-Ile-L-Glu-Gly-L-Arg-4-nitroanilide + H2O <=> benzoyl-L-Ile-L-Glu-Gly-L-Arg + 4-nitroaniline 3.4.21.B12

Suc-Phe-4-nitroanilide + H2O <=> carboxypropionyl-Phe + 4-nitroaniline 3.4.16.5 carboxypeptidase C

DRRETLFQ-4-nitroanilide + H2O <=> DRRETLFG + 4-nitroaniline 3.4.22.28 picornain 3C

DSLETLFQ-4-nitroanilide + H2O <=> DSLETLFG + 4-nitroaniline 3.4.22.28 picornain 3C

DSLEVLFQ-4-nitroanilide + H2O <=> DSLEVLFG + 4-nitroaniline 3.4.22.28 picornain 3C

DSRETLFQ-4-nitroanilide + H2O <=> DSRETLFG + 4-nitroaniline 3.4.22.28 picornain 3C

EALFQ-4-nitroanilide + H2O <=> EALFG + 4-nitroaniline 3.4.22.28 picornain 3C

ETLFQ-4-nitroanilide + H2O <=> ETLFG + 4-nitroaniline 3.4.22.28 picornain 3C

EVLFQ-4-nitroanilide + H2O <=> EVLFG + 4-nitroaniline 3.4.22.28 picornain 3C

DEVD-4-nitroanilide + H2O <=> DEVD + 4-nitroaniline 3.4.22.56 caspase-3
3.4.22.60 caspase-7
3.4.22.63 caspase-10



Lys-Val-Tyr_4-nitroanilide + H2O <=> Lys-Val-Tyr + 4-nitroaniline 3.4.21.77 semenogelase

methoxysuccinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O <=> methoxy-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme

Nle-4-nitroanilide + H2O <=> Nle + 4-nitroaniline 3.4.11.18 methionyl aminopeptidase

succinyl-Phe-Leu-Phe-4-nitroanilide + H2O <=> succinyl-Phe-Leu-Phe + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme

TLFQ-4-nitroanilide + H2O <=> TLFG + 4-nitroaniline 3.4.22.28 picornain 3C

alpha-N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O <=> N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline 3.4.21.B3

succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide + H2O <=> N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline 3.4.21.1 chymotrypsin

Suc-Ala-Ala-Pro-Phe-p-nitroanilide + H2O <=> N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline 3.4.21.111 aqualysin 1
3.4.21.25 cucumisin
3.4.21.B21



N-glutaryl-L-Phe-p-nitroanilide + H2O <=> N-glutaryl-L-Phe + 4-nitroaniline 3.4.21.1 chymotrypsin

succinyl-L-Ala-L-Ala-L-Pro-L-Tyr-4-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Tyr + 4-nitroaniline 3.4.21.2 chymotrypsin C
3.4.21.62 subtilisin


succinimidyl-Ala-Ala-Pro-Phe-p-nitroanilide + H2O <=> succinimidyl-Ala-Ala-Pro-Phe + 4-nitroaniline 3.4.21.1 chymotrypsin

N-succinyl-Ala-Pro-Ala-4-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Ala + 4-nitroaniline 3.4.21.62 subtilisin

succinyl-AAPA-p-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Ala + 4-nitroaniline 3.4.21.12 alpha-lytic endopeptidase
3.4.21.2 chymotrypsin C


Suc-AAPV-4-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Val + 4-nitroaniline 3.4.21.76 myeloblastin

succinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Val + 4-nitroaniline 3.4.21.12 alpha-lytic endopeptidase
3.4.21.2 chymotrypsin C


N-succinyl-Ala-Ala-Pro-Nle-p-nitroanilide + H2O <=> N-succinyl-Ala-Ala-Pro-Nle + 4-nitroaniline 3.4.21.1 chymotrypsin

N-succinyl-Ala-Ala-Pro-Nva-p-nitroanilide + H2O <=> N-succinyl-Ala-Ala-Pro-Nva + 4-nitroaniline 3.4.21.1 chymotrypsin

benzyloxycarbonyl-Ala-Ala-Met-Glu-4-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Ala-Met-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

benzyloxycarbonyl-Ala-Ala-Trp-Glu-4-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Ala-Trp-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

benzyloxycarbonyl-Ala-Ala-Phe-Glu-4-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Ala-Phe-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

benzyloxycarbonyl-L-Ala-L-Ala-L-Leu-L-Glu-4-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Ala-Leu-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

benzyloxycarbonyl-Ala-Ala-Leu-Glu-4-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Ala-Leu-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

benzyloxycarbonyl-Gly-Ala-Ala-Glu-4-nitroanilide + H2O <=> benzyloxycarbonyl-Gly-Ala-Ala-Glu + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

benzyloxycarbonyl-Ala-Ala-Trp-Asp-4-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Ala-Trp-Asp + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

benzyloxycarbonyl-Ala-Ala-Leu-Asp-4-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Ala-Leu-Asp + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

benzyloxycarbonyl-Ala-Ala-Phe-Asp-4-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Ala-Phe-Asp + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

benzyloxycarbonyl-Ala-Ala-Met-Asp-4-nitroanilide + H2O <=> benzyloxycarbonyl-Ala-Ala-Met-Asp + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

benzyloxycarbonyl-Gly-Ala-Ala-Asp-4-nitroanilide + H2O <=> benzyloxycarbonyl-Gly-Ala-Ala-Asp + 4-nitroaniline 3.4.21.19 glutamyl endopeptidase

4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide + H2O <=> CH3SO2-D-Leu-Gly-L-Arg + 4-nitroaniline 3.4.21.22 coagulation factor IXa

succinyl-D-Ala-Pro-4-nitroanilide + H2O <=> benzyloxycarbonyl-D-Ala-Pro + 4-nitroaniline 3.4.21.26 prolyl oligopeptidase

Ac-AGLTR-4-nitroanilide + H2O <=> Ac-AGLTR + 4-nitroaniline 3.4.21.34 plasma kallikrein

Glu-Pro-Arg-p-nitroanilide + H2O <=> Glu-Pro-Arg + 4-nitroaniline 3.4.21.34 plasma kallikrein
3.4.21.42 complement subcomponent C_overbar_1s_


succinyl-Ala-Ala-Pro-Trp_4-nitroanilide + H2O <=> succinyl-Ala-Ala-Pro-Trp + 4-nitroaniline 3.4.21.2 chymotrypsin C

Boc-L-Lys-4-nitroanilide + H2O <=> tert-butyloxycarbonyl-Lys + 4-nitroaniline 3.4.11.2 membrane alanyl aminopeptidase

D-phenylalanyl-pipecolyl-L-arginine-4-nitroanilide + H2O <=> D-phenylalanyl-pipecolyl-L-arginine + 4-nitroaniline 3.4.21.5 thrombin
3.4.21.59 tryptase


D-Phe-Pro-Lys-4-nitroanilide + H2O <=> D-Phe-Pro-Lys + 4-nitroaniline 3.4.21.5 thrombin

succinyl-Ala-Ala-Ala-Ala-Ala-p-nitroanilide + H2O <=> succinyl-AAAAA + 4-nitroaniline 3.4.21.62 subtilisin

dimethylsulfonyl-D-norleucine-Gly-Arg-4-nitroanilide + H2O <=> dimethylsulfonyl-D-norleucine-Gly-Arg + 4-nitroaniline 3.4.21.6 coagulation factor Xa

methoxycarbonyl-D-cyclohexylglycyl-glycyl-L-ariginine-4-nitroanilide + H2O <=> methoxycarbonyl-D-cyclohexylglycyl-glycyl-L-ariginine + 4-nitroaniline 3.4.21.6 coagulation factor Xa

methoxycarbonyl-D-cyclohexylglycyl-glycyl-L-arginyl-p-nitroanilide + H2O <=> methoxycarbonyl-D-cyclohexylglycyl-glycyl-L-ariginine + 4-nitroaniline 3.4.21.22 coagulation factor IXa

N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine-4-nitroanilide + H2O <=> N-alpha-benzyloxycarbonyl-D-arginyl-glycyl-L-arginine + 4-nitroaniline 3.4.21.21 coagulation factor VIIa
3.4.21.22 coagulation factor IXa
3.4.21.27 coagulation factor XIa
3.4.21.6 coagulation factor Xa
3.4.21.68 t-plasminogen activator





S2222 + H2O <=> benzoyl-Ile-Glu(gamma-OR)-Gly-Arg + 4-nitroaniline 3.4.21.6 coagulation factor Xa
3.4.21.B1


tert-butoxycarbonyl-Gly-Gly-Arg-p-nitroanilide + H2O <=> tert-butyloxycarbonyl-Gly-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

benzyloxycarbonyl-D-Phe-Gly-Arg-p-nitroanilide + H2O <=> benzyloxycarbonyl-D-Phe-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

benzyloxycarbonyl-Asp-Gly-Arg-p-nitroanilide + H2O <=> benzyloxycarbonyl-Asp-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

benzyloxycarbonyl-D-Asp(O-tert-butyl)-Gly-Arg-p-nitroanilide + H2O <=> benzyloxycarbonyl-Asp(O-tert-butyl)-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

benzyloxycarbonyl-D-Asp(O-tert-butyl)-Gly-Arg-p-nitroanilide + H2O <=> benzyloxycarbonyl-D-Asp(O-tert-butyl)-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

benzyloxycarbonyl-Glu-(O-tert-butyl)Gly-Arg-p-nitroanilide + H2O <=> benzyloxycarbonyl-Glu(O-tert-butyl)-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

benzyloxycarbonyl-pyroglutamic_acid-Gly-Arg-p-nitroanilide + H2O <=> benzyloxycarbonyl-pyroglutamic_acid-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

methyloxycarbonyl-D-Leu-Gly-Arg-p-nitroanilide + H2O <=> methyloxycarbonyl-D-Leu-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

methyloxycarbonyl-D-hexahydrotyrosyl-Gly-Arg-p-nitroanilide + H2O <=> methyloxycarbonyl-D-hexahydrotyrosyl-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

D-Phe-Gly-Arg-p-nitroanilide + H2O <=> D-Phe-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

cyclohexylglycyl-Gly-Arg-p-nitroanilide + H2O <=> cyclohexylglycyl-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

D-hexahydrotyrosyl-Gly-Arg-p-nitroanilide + H2O <=> D-hexahydrotyrosyl-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

Asp-Gly-Arg-p-nitroanilide + H2O <=> Asp-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

D-Asp-Gly-Arg-p-nitroanilide + H2O <=> D-Asp-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

D-Lys-Gly-Arg-p-nitroanilide + H2O <=> D-Lys-Gly-Arg + 4-nitroaniline 3.4.21.68 t-plasminogen activator

DL-pyroglutamic_acid-cyclohexylglycine-Arg_4-nitroanilide + H2O <=> DL-pyroglutamic_acid-cyclohexylglycine-Arg + 4-nitroaniline 3.4.21.B1

D-cyclohexylglycine-Ala-Arg-4-nitroanilide + H2O <=> D-cyclohexylglycine-Ala-Arg + 4-nitroaniline 3.4.21.5 thrombin
3.4.21.B1


methyloxycarbonyl-D-cyclohexylalanyl-Gly-Arg-p-nitroanilide + H2O <=> CH3-OCO-D-cyclohexylalanine-Gly-Arg + 4-nitroaniline 3.4.21.B1
3.4.21.B60


D-Lys(benzyloxycarbonyl)-Pro-Arg-4-nitroanilide + H2O <=> D-Lys(benzyloxycarbonyl)-Pro-Arg + 4-nitroaniline 3.4.21.B1

CH2SO2-D-cyclohexylalanine-butanoyl-Arg-4-nitroanilide + H2O <=> CH2SO2-D-cyclohexylalanine-butanoyl-Arg + 4-nitroaniline 3.4.21.B1

methylsulfonyl-D-cyclohexylglycyl-Gly-Arg-p-nitroanilide + H2O <=> CH2SO2-D-cyclohexylglycine-Gly-Arg + 4-nitroaniline 3.4.21.22 coagulation factor IXa
3.4.21.27 coagulation factor XIa


methylsulfonyl-D-cyclohexyltyrosylglycyl-arginine-p-nitroanilide + H2O <=> CH2SO2-D-cyclohexylglycine-Gly-Arg + 4-nitroaniline 3.4.21.109 matriptase
3.4.21.68 t-plasminogen activator


CH2SO2-D-cyclohexylglycine-Gly-Arg-4-nitroanilide <=> CH2SO2-D-cyclohexylglycine-Gly-Arg + 4-nitroaniline 3.4.21.B1

D-Ala-cyclohexyltyrosine-Lys-4-nitroanilide + H2O <=> D-Ala-cyclohexyltyrosine-Lys + 4-nitroaniline 3.4.21.B1

D-Ala-Pro-Arg-4-nitroanilide + H2O <=> D-Ala-Pro-Arg + 4-nitroaniline 3.4.22.26 cancer procoagulant

Glu-Glu-Pro-Arg-4-nitroanilide + H2O <=> Glu-Glu-Pro-Arg + 4-nitroaniline 3.4.22.26 cancer procoagulant

methylsulfonyl-D-cyclohexylalanyl-butyl-Arg-4-nitroanilide + H2O <=> methylsulfonyl-D-cyclohexylalanyl-butyl-Arg + 4-nitroaniline 3.4.22.26 cancer procoagulant

sarcosyl-Pro-Arg-4-nitroanilide + H2O <=> sarcosyl-Pro-Arg + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_
3.4.22.26 cancer procoagulant


Leu-Pro-Arg-4-nitroanilide + H2O <=> Leu-Pro-Arg + 4-nitroaniline 3.4.22.26 cancer procoagulant

Glu-Glu-Phe-Lys-4-nitroanilide + H2O <=> Glu-Glu-Phe-Lys + 4-nitroaniline 3.4.22.26 cancer procoagulant

benzyloxycarbonyl-Leu-Pro-Arg-4-nitroanilide + H2O <=> benzyloxycarbonyl-Leu-Pro-Arg + 4-nitroaniline 3.4.22.26 cancer procoagulant

pyr-Glu-Phe-Leu-4-nitroanilide + H2O <=> pyr-Glu-Phe-Leu + 4-nitroaniline 3.4.22.30 caricain
3.4.22.31 ananain


Pyr-Phe-Lys-4-nitroanilide + H2O <=> Pyr-Phe-Lys + 4-nitroaniline 3.4.22.32 stem bromelain

Leu-Tyr-Arg-4-nitroanilide + H2O <=> LYR + 4-nitroaniline 3.4.22.37 gingipain R

N-tert-butyloxycarbonyl-Val-Leu-Gly-Arg-4-nitroanilide + H2O <=> butoxy-carbonyl-Val-Leu-Gly-Arg + 4-nitroaniline 3.4.22.37 gingipain R

butoxy-carbonyl-O-benzyl-Ser-Gly-Arg-4-nitroanilide + H2O <=> butoxy-carbonyl-O-benzyl-Ser-Gly-Arg + 4-nitroaniline 3.4.22.37 gingipain R

5-L-glutamyl-4-nitroanilide + L-isoleucine <=> 4-nitroaniline + gamma-L-Glu-L-isoleucine 2.3.2.2 gamma-glutamyltransferase

succinyl-Phe-Ala-Ala-Phe-p-nitroanilide + H2O <=> succinyl-Phe-Ala-Ala-Phe + 4-nitroaniline 3.4.21.62 subtilisin

benzoyl-Ile-Glu-gamma-methoxyglutamyl-Gly-Arg-p-nitroanilide + H2O <=> benzoyl-Ile-Glu-gamma-methoxyglutamyl-Gly-Arg + 4-nitroaniline 3.4.21.21 coagulation factor VIIa

pyroglutamyl-Phe-Lys-p-nitroanilide + H2O <=> pyroglutamyl-D-Phe-Gly-Arg + 4-nitroaniline 3.4.21.21 coagulation factor VIIa

5-L-glutamyl-4-nitroanilide + L-tyrosine <=> 4-nitroaniline + 5-L-glutamyl-L-Tyr 2.3.2.2 gamma-glutamyltransferase

N-succinyl-Ala-Ala-Val_4-nitroanilide + H2O <=> N-succinyl-Ala-Ala-Val + 4-nitroaniline 3.4.21.111 aqualysin 1
3.4.21.37 leukocyte elastase


N-succinyl-Phe-Nle-Ala-4-nitroanilide + H2O <=> N-succinyl-Phe-Nle-Ala + 4-nitroaniline 3.4.21.111 aqualysin 1

N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O <=> N-succinyl-Phe-Ala-Ala + 4-nitroaniline 3.4.21.111 aqualysin 1

N-succinyl-Phe-Leu-Ala-4-nitroanilide + H2O <=> N-succinyl-Phe-Leu-Ala + 4-nitroaniline 3.4.21.111 aqualysin 1

N-succinyl-Phe-Val-Ala-4-nitroanilide + H2O <=> N-succinyl-Phe-Val-Ala + 4-nitroaniline 3.4.21.111 aqualysin 1

N-succinyl-Gly-Gly-Phe-4-nitroanilide + H2O <=> N-succinyl-Gly-Gly-Phe + 4-nitroaniline 3.4.19.1 acylaminoacyl-peptidase
3.4.21.111 aqualysin 1
3.4.21.39 chymase
3.4.21.B3
3.4.21.B52





N-succinyl-Phe-Val-Leu-4-nitroanilide + H2O <=> N-succinyl-Phe-Val-Leu + 4-nitroaniline 3.4.21.111 aqualysin 1

N-succinyl-Phe-Val-Phe-4-nitroanilide + H2O <=> N-succinyl-Phe-Val-Phe + 4-nitroaniline 3.4.21.111 aqualysin 1

N-succinyl-Ile-Val-Ala-4-nitroanilide + H2O <=> N-succinyl-Ile-Val-Ala + 4-nitroaniline 3.4.21.111 aqualysin 1

N-succinyl-Phe-betaAla-Ala-4-nitroanilide + H2O <=> N-succinyl-Phe-betaAla-Ala + 4-nitroaniline 3.4.21.111 aqualysin 1

N-succinyl-Gly-betaAla-Ala-4-nitroanilide + H2O <=> N-succinyl-Gly-betaAla-Ala + 4-nitroaniline 3.4.21.111 aqualysin 1

N-succinyl-Leu-betaAla-Ala-4-nitroanilide + H2O <=> N-succinyl-Leu-betaAla-Ala + 4-nitroaniline 3.4.21.111 aqualysin 1

N-succinyl-Ile-betaAla-Ala-4-nitroanilide + H2O <=> N-succinyl-Ile-betaAla-Ala + 4-nitroaniline 3.4.21.111 aqualysin 1

N-succinyl-Ala-Ala-Val-Ala-4-nitroanilide + H2O <=> 4-nitroaniline + Suc-Ala-Ala-Val-Ala 3.4.21.111 aqualysin 1
3.4.21.62 subtilisin
3.4.21.71 pancreatic elastase II



alpha-N-succinyl-Ser-Pro-Lys-4-nitroanilide + H2O <=> alpha-N-succinyl-Ser-Pro-Lys + 4-nitroaniline 3.4.21.B3

alpha-N-succinyl-Thr-Pro-Lys-4-nitroanilide + H2O <=> alpha-N-succinyl-Thr-Pro-Lys + 4-nitroaniline 3.4.21.B3

Suc-Ala-Ala-Pro-Lys-p-nitroanilide + H2O <=> Suc-Ala-Ala-Pro-Lys + 4-nitroaniline 3.4.21.109 matriptase

5-L-glutamyl-4-nitroanilide + L-arginine <=> 4-nitroaniline + 5-L-glutamyl-L-Arg 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-asparagine <=> 4-nitroaniline + 5-L-glutamyl-L-Asn 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + Met-NH2 <=> 4-nitroaniline + 5-L-glutamyl-L-methioninamide 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-methionyl(2,2,2-trifluoroethylamide) <=> 4-nitroaniline + 5-L-glutamyl-L-methionyl(2,2,2-trifluoroethylamide) 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-methionyl(2-fluoroethylamide) <=> 4-nitroaniline + 5-L-glutamyl-L-methionyl(2-fluoroethylamide) 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-methionyl(3,3,3-trifluoropropylamide) <=> 4-nitroaniline + 5-L-glutamyl-L-methionyl(3,3,3-trifluoropropylamide) 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-methionylethylamide <=> 4-nitroaniline + 5-L-glutamyl-L-methionylethylamide 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-methionylpropylamide <=> 4-nitroaniline + 5-L-glutamyl-L-methionylpropylamide 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-proline <=> 4-nitroaniline + 5-L-glutamyl-L-Pro 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + taurine <=> 4-nitroaniline + 5-L-glutamyl-taurine 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + glycine <=> 4-nitroaniline + 5-L-Glutamyl-glycine 2.3.2.2 gamma-glutamyltransferase

Nalpha-benzoyl-Ala_4-nitroanilide + H2O <=> Benzoyl-Ala + 4-nitroaniline 3.4.21.57 leucyl endopeptidase

benzyloxycarbonyl-Leu-4-nitroanilide + H2O <=> Benzyloxycarbonyl-Leu + 4-nitroaniline 3.4.21.B52

N-benzyloxycarbonyl-L-Pro_4-nitroanilide + H2O <=> Benzyloxycarbonyl-Pro + 4-nitroaniline 3.4.16.2 lysosomal Pro-Xaa carboxypeptidase

D-Ile-Pro-Arg-p-nitroanilide + H2O <=> D-Ile-Pro-Arg + 4-nitroaniline 3.4.21.21 coagulation factor VIIa
3.4.21.68 t-plasminogen activator
3.4.21.B39
3.4.21.B45




Peptide_S-2288 + H2O <=> D-Ile-Pro-Arg + 4-nitroaniline 3.4.21.21 coagulation factor VIIa

D-Ile-Pro-Arg-4-nitroanilide + H2O <=> D-Ile-Pro-Arg + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme
3.4.21.21 coagulation factor VIIa
3.4.21.27 coagulation factor XIa
3.4.21.35 tissue kallikrein
3.4.21.59 tryptase
3.4.21.68 t-plasminogen activator
3.4.21.73 u-plasminogen activator
3.4.22.26 cancer procoagulant
3.4.24.72 fibrolase









acetyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O <=> acetyl-Ala-Ala-Pro-Val + 4-nitroaniline 3.4.21.37 leukocyte elastase

L-Asp-L-Ala-L-Phe-4-nitroanilide + H2O <=> Asp-Ala-Phe + 4-nitroaniline 3.4.14.9 tripeptidyl-peptidase I

L-Ala-Ala-Pro-Leu-4-nitroanilide + H2O <=> L-Ala-Ala-Pro-Leu + 4-nitroaniline 3.4.11.22 aminopeptidase I
3.4.11.6 aminopeptidase B
3.4.21.39 chymase
3.4.21.62 subtilisin




N-methoxysuccinyl-Ala-Ala-Pro-Val-4-nitroanilide + H2O <=> MeO-Suc-Ala-Ala-Pro-Val + 4-nitroaniline 3.4.21.76 myeloblastin

MeOSuc-AIPM-4-nitroanilide + H2O <=> MeOSuc-AIPM + 4-nitroaniline 3.4.21.76 myeloblastin

Suc-Ala-Ala-p-nitroanilide + H2O <=> succinyl-Ala-Ala-NH2 + 4-nitroaniline 3.4.21.37 leukocyte elastase

His-Ala-p-nitroanilide + H2O <=> His-Ala + 4-nitroaniline 3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV


N-succinyl-L-Leu-L-Leu-L-Val-L-Tyr-4-nitroanilide + H2O <=> Succinyl-Leu-Leu-Val-Tyr + 4-nitroaniline 3.4.21.20 cathepsin G
3.4.21.39 chymase


Tyr-Ala-p-nitroanilide + H2O <=> Tyr-Ala + 4-nitroaniline 3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV


4-methylsulfonyl-D-Leu-Gly-Arg-p-nitroanilide + H2O <=> 4-methylsulfonyl-D-Leu-Gly-Arg + 4-nitroaniline 3.4.21.22 coagulation factor IXa

acetyl-L-Asp-L-Met-L-Gln-L-Asp-4-nitroanilide + H2O <=> acetyl-L-Asp-L-Met-L-Gln-L-Asp + 4-nitroaniline 3.4.22.56 caspase-3

Ac-DVAD-p-nitroanilide + H2O <=> 4-nitroaniline + acetyl-L-Asp-L-Val-L-Ala-L-Asp 3.4.22.56 caspase-3
3.4.22.60 caspase-7


Ac-LDVAD-p-nitroanilide + H2O <=> 4-nitroaniline + acetyl-L-Leu-L-Asp-L-Val-L-Ala-L-Asp 3.4.22.56 caspase-3
3.4.22.60 caspase-7


benzyloxycarbonyl-D-arginyl-glycyl-arginine-p-nitroanilide + H2O <=> benzyloxycarbonyl-D-arginyl-glycyl-arginine + 4-nitroaniline 3.4.21.21 coagulation factor VIIa

Bz-Ile-Glu-Arg-4-nitroanilide + H2O <=> Bz-Ile-Glu-Arg + 4-nitroaniline 3.4.22.26 cancer procoagulant

D-Phe-Pro-Phe-4-nitroanilide + H2O <=> D-Phe-L-Pro-L-Phe + 4-nitroaniline 3.4.21.5 thrombin

Peptide_S-2288 + H2O <=> H-D-isoleucyl-L-prolyl-arginine + 4-nitroaniline 3.4.21.42 complement subcomponent C_overbar_1s_

D-Ile-Pro-Arg-4-nitroanilide + H2O <=> H-D-isoleucyl-L-prolyl-arginine + 4-nitroaniline 3.4.21.21 coagulation factor VIIa

S-2288 + H2O <=> H-D-isoleucyl-L-prolyl-arginine + 4-nitroaniline 3.4.21.59 tryptase

L-Lys-L-Pro-L-Pro-4-nitroanilide + H2O <=> L-Pro-L-Pro-L-Pro + 4-nitroaniline 3.4.14.12 Xaa-Xaa-Pro tripeptidyl-peptidase

Nalpha-benzoyl-DL-lysine_p-nitroanilide + H2O <=> N-alpha-benzoyl-DL-Lys + 4-nitroaniline 3.4.22.37 gingipain R
3.4.22.47 gingipain K


N-butyl-(R)-cyclohexylalanyl-L-Arg-p-nitroanilide + H2O <=> N-butyl-(R)-cyclohexylalanyl-L-Arg + 4-nitroaniline 3.4.21.34 plasma kallikrein

N-methylsulfonyl-D-phenyl-D-glycyl-D-arginyl-4-nitroanilide + H2O <=> N-methylsulfonyl-D-phenyl-D-glycyl-D-arginine + 4-nitroaniline 3.4.21.21 coagulation factor VIIa

succinyl-L-Ala-L-Ala-L-Pro-Gly-4-nitroanilide + H2O <=> succinyl-L-Ala-L-Ala-L-Pro-Gly + 4-nitroaniline 3.4.21.62 subtilisin

benzyloxycarbonyl-D-arginyl-D-glycyl-D-arginine-p-nitroanilide + H2O <=> 4-nitroaniline + benzyloxycarbonyl-D-arginyl-D-glycyl-D-arginine 3.4.21.21 coagulation factor VIIa

N-Glu-L-Phe-4-nitroanilide + H2O <=> 4-nitroaniline + N-glutamyl-L-phenylalanine 3.4.21.1 chymotrypsin

Gly-Pro-Leu-p-nitroanilide + H2O <=> Gly-Pro-Leu + 4-nitroaniline 3.5.1.13 aryl-acylamidase

phthalyl-Phe-Leu-p-nitroanilide + H2O <=> phthalyl-Phe-Leu + 4-nitroaniline 3.4.22.2 papain

5-L-glutamyl-4-nitroanilide + Ala-Ala-Ala <=> 4-nitroaniline + 5-L-glutamyl-Ala-Ala-Ala 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + DL-phenylalanine <=> 4-nitroaniline + 5-L-glutamyl-DL-Phe 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + Gly-Gly-Gly <=> 4-nitroaniline + 5-L-glutamyl-Gly-Gly-Gly 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-histidine <=> 4-nitroaniline + 5-L-glutamyl-His 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-isoleucine <=> 4-nitroaniline + 5-L-glutamyl-Ile 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-threonine <=> 4-nitroaniline + 5-L-glutamyl-Thr 2.3.2.2 gamma-glutamyltransferase

N-acetyl-Met-p-nitroanilide + H2O <=> N-acetyl-L-methionine + 4-nitroaniline 3.4.19.1 acylaminoacyl-peptidase

Met-Pro-p-nitroanilide + H2O <=> Met-Pro + 4-nitroaniline 3.4.13.18 cytosol nonspecific dipeptidase
3.4.14.5 dipeptidyl-peptidase IV


L-Met-L-Pro-4-nitroanilide + H2O <=> Met-Pro + 4-nitroaniline 3.4.14.5 dipeptidyl-peptidase IV

N-acetyl-Asp-Glu-Val-Asp-p-nitroanilide + H2O <=> acetyl-DEVD + 4-nitroaniline 3.4.22.55 caspase-2
3.4.22.56 caspase-3
3.4.22.60 caspase-7
3.4.22.62 caspase-9




acetyl-DEVD-4-nitroanilide + H2O <=> acetyl-DEVD + 4-nitroaniline 3.4.22.36 caspase-1
3.4.22.56 caspase-3
3.4.22.57 caspase-4
3.4.22.59 caspase-6
3.4.22.60 caspase-7
3.4.22.61 caspase-8






acetyl-Ile-Glu-Thr-Asp-p-nitroanilide + H2O <=> acetyl-IETD-7 + 4-nitroaniline 3.4.22.61 caspase-8
3.4.22.62 caspase-9


acetyl-Trp-Glu-His-Asp-p-nitroanilide + H2O <=> acetyl-Trp-Glu-His-Asp + 4-nitroaniline 3.4.22.62 caspase-9

acetyl-Val-Glu-Ile-Asp-p-nitroanilide + H2O <=> acetyl-Val-Glu-Ile-Asp + 4-nitroaniline 3.4.22.62 caspase-9

Ala-Ala-Pro-Met-4-nitroanilide + H2O <=> Ala-Ala-Pro-Met + 4-nitroaniline 3.4.21.39 chymase
3.4.21.62 subtilisin


L-diglutamyl-glycyl-L-arginine_4-nitroanilide + H2O <=> diglutamyl-Gly-Arg + 4-nitroaniline 3.4.21.73 u-plasminogen activator

L-Pyroglutamyl-L-Phe-L-Leu_4-nitroanilide + H2O <=> L-pyroglutamyl-L-Phe-Leu + 4-nitroaniline 3.4.22.15 cathepsin L
3.4.22.7 asclepain
3.4.22.B5



L-Pro-Phe-Leu-4-nitroanilide + H2O <=> L-Pro-Phe-Leu + 4-nitroaniline 3.4.22.2 papain

methoxy-succinyl-Arg-Ala-Tyr-4-nitroanilide + H2O <=> methoxy-succinyl-Arg-Ala-Tyr + 4-nitroaniline 3.4.21.39 chymase

succinyl-Ala-(cis)-Pro-Phe-NH-4-nitroanilide + H2O <=> N-succinyl-Ala-Pro-Phe + 4-nitroaniline 3.4.21.1 chymotrypsin

succinyl-Ala-Ala-Pro-4-nitroanilide + H2O <=> N-succinyl-L-Ala-Ala-Pro + 4-nitroaniline 3.4.21.1 chymotrypsin

succinyl-Phe-Arg-p-nitroanilide + H2O <=> succinyl-Phe-Arg + 4-nitroaniline 3.4.22.6 chymopapain

succinyl-Phe-Leu-p-nitroanilide + H2O <=> succinyl-Phe-Leu + 4-nitroaniline 3.4.22.2 papain

tert-butyloxycarbonyl-Ala-Ala-Gly_4-nitroanilide + H2O <=> tert-butyloxycarbonyl-Ala-Ala-Gly + 4-nitroaniline 3.4.22.25 glycyl endopeptidase
3.4.22.3 ficain


Trp-Glu-His-Asp-p-nitroanilide + H2O <=> Trp-Glu-His-Asp + 4-nitroaniline 3.4.22.36 caspase-1
3.4.22.58 caspase-5


D-Glu-Phe-Lys-p-nitroanilide + H2O <=> D-Glu-Phe-Lys + 4-nitroaniline 3.4.21.68 t-plasminogen activator

acetyl-IEPD-p-nitroanilide + H2O <=> N-acetyl-Ile-Glu-Pro-Asp + 4-nitroaniline 3.4.21.78 granzyme A
3.4.21.79 granzyme B


N-Suc-Gly-Phe-Gly-p-nitroanilide + H2O <=> N-Suc-Gly-Phe-Gly + 4-nitroaniline 3.4.22.48 staphopain

succinyl-Ala-Val-Pro-Phe-4-nitroanilide + H2O <=> succinyl-Ala-Val-Pro-Phe + 4-nitroaniline 3.4.21.117 stratum corneum chymotryptic enzyme

benzyloxycarbonyl-L-alanine-X-L-arginine-p-nitroanilide + H2O <=> 4-nitroaniline + benzyloxycarbonyl-L-alanine-X-L-arginine 3.4.21.9 enteropeptidase

formyl-Ala-Phe-Arg-4-nitroanilide + H2O <=> 4-nitroaniline + formyl-Ala-Phe-Arg 3.4.21.9 enteropeptidase

formyl-Ala-Phe-Lys-4-nitroanilide + H2O <=> 4-nitroaniline + formyl-Ala-Phe-Lys 3.4.21.9 enteropeptidase

Z-Ala-Met-Arg-nitroanilide + H2O <=> 4-nitroaniline + Z-Ala-Met-Arg 3.4.21.9 enteropeptidase

Z-Ala-Phe-Arg-nitroanilide + H2O <=> 4-nitroaniline + Z-Ala-Phe-Arg 3.4.21.9 enteropeptidase

Z-Ala-Tyr-Arg-nitroanilide + H2O <=> 4-nitroaniline + Z-Ala-Tyr-Arg 3.4.21.9 enteropeptidase

Ac-Lys_4-nitrophenyl_amide + H2O <=> N-alpha-acetyl-L-lysine + 4-nitroaniline 3.4.22.47 gingipain K
3.4.22.8 clostripain


acetyl-Lys-p-nitroaniline + H2O <=> N-alpha-acetyl-L-lysine + 4-nitroaniline 3.4.21.50 lysyl endopeptidase

N-alpha-acetyl-L-lysine-p-nitroanilide + H2O <=> N-alpha-acetyl-L-lysine + 4-nitroaniline 3.4.22.47 gingipain K

5-L-glutamyl-4-nitroanilide + L-cysteine <=> 4-nitroaniline + gamma-Glu-L-Cys 2.3.2.2 gamma-glutamyltransferase

Nalpha-benzoyl-Gly_4-nitroanilide + H2O <=> hippuric_acid + 4-nitroaniline 3.4.21.57 leucyl endopeptidase

acetyl-Glu-Lys-Arg-p-nitroanilide + H2O <=> acetyl-Glu-Lys-Arg + 4-nitroaniline 3.4.21.35 tissue kallikrein

L-cystinyl-p-nitroanilide + H2O <=> L-cystine + 4-nitroaniline 3.4.11.10 bacterial leucyl aminopeptidase

Cystinyl_4-nitroanilide + H2O <=> L-cystine + 4-nitroaniline 3.4.11.22 aminopeptidase I
3.4.11.3 cystinyl aminopeptidase


N-succinyl-L-Phe-p-nitroanilide + H2O <=> N-succinyl-L-phenylalanine + 4-nitroaniline 3.4.21.1 chymotrypsin

Suc-Phe-4-nitroanilide + H2O <=> N-succinyl-L-phenylalanine + 4-nitroaniline 3.4.21.1 chymotrypsin
3.4.21.B52


N-succinyl-L-Phe-4-nitroanilide + H2O <=> N-succinyl-L-phenylalanine + 4-nitroaniline 3.4.21.64 peptidase K

Gly-L-Pro-4-nitroanilide + H2O <=> Gly-Pro + 4-nitroaniline 3.4.14.11 Xaa-Pro dipeptidyl-peptidase
3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV
3.4.21.26 prolyl oligopeptidase




Asn-Pro-p-nitroanilide + H2O <=> L-Asn-L-Pro + 4-nitroaniline 3.4.14.5 dipeptidyl-peptidase IV

methoxycarbonylcyclohexyl-Gly-Gly-L-Arg-4-nitroanilide + H2O <=> methoxycarbonylcyclohexyl-Gly-Gly-L-Arg + 4-nitroaniline 3.4.21.6 coagulation factor Xa

methoxysuccinyl-Arg-Pro-Tyr-4-nitroanilide + H2O <=> N-methoxysuccinyl-Arg-Pro-Tyr + 4-nitroaniline 3.4.21.35 tissue kallikrein

N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O <=> N-methoxysuccinyl-Ala-Ala-Ala + 4-nitroaniline 3.4.21.37 leukocyte elastase

L-glutamyl_4-nitroanilide + H2O <=> L-glutamine + 4-nitroaniline 3.4.11.B9

5-L-glutamyl-4-nitroanilide + H2O <=> L-glutamine + 4-nitroaniline 2.6.1.16 glutamine---fructose-6-phosphate transaminase (isomerizing)

Boc-Ala-Ala-Pro-Ala-p-nitroanilide + H2O <=> tert-butyloxycarbonyl-Ala-Ala-Pro + L-alanine + 4-nitroaniline 3.4.21.26 prolyl oligopeptidase

L-Ala-4-nitroanilide + H2O <=> L-alanine + 4-nitroaniline 3.3.2.6 leukotriene-A4 hydrolase
3.4.11.1 leucyl aminopeptidase
3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.19 D-stereospecific aminopeptidase
3.4.11.2 membrane alanyl aminopeptidase
3.4.11.20 aminopeptidase Ey
3.4.11.22 aminopeptidase I
3.4.11.24 aminopeptidase S
3.4.11.6 aminopeptidase B
3.4.13.20 beta-Ala-His dipeptidase
3.4.21.26 prolyl oligopeptidase
3.5.1.13 aryl-acylamidase












L-alanine-4-nitroanilide + H2O <=> L-alanine + 4-nitroaniline 3.3.2.6 leukotriene-A4 hydrolase
3.4.11.1 leucyl aminopeptidase
3.4.11.24 aminopeptidase S



L-phenylalanyl_4-nitroanilide + H2O <=> L-phenylalanine + 4-nitroaniline 3.4.11.B3
3.4.11.B9


Phe-p-nitroanilide + H2O <=> L-phenylalanine + 4-nitroaniline 3.4.19.1 acylaminoacyl-peptidase
3.4.22.40 bleomycin hydrolase


D-Phe-Pro-Phe-4-nitroanilide + H2O <=> D-Phe-Pro-Phe + 4-nitroaniline 3.4.21.5 thrombin

Glu-Glu-Pro-Arg-4-nitroanilide + H2O <=> di-L-Glu-L-Pro-L-Arg + 4-nitroaniline 3.4.21.27 coagulation factor XIa

di-L-Glu-L-Pro-L-Arg-4-nitroanilide + H2O <=> di-L-Glu-L-Pro-L-Arg + 4-nitroaniline 3.4.21.5 thrombin

glutaryl-L-alanyl-L-alanyl-L-prolyl-L-leucyl-4-nitroanilide + H2O <=> glutaryl-Ala-Ala-Pro-Leu + 4-nitroaniline 3.4.21.25 cucumisin

glutaryl-Ala-Ala-Pro-Leu-p-nitroanilide + H2O <=> glutaryl-Ala-Ala-Pro-Leu + 4-nitroaniline 3.4.21.62 subtilisin

Thr-Ser-Ala-Val-Leu-Gln-p-nitroanilide + H2O <=> L-Thr-L-Ser-L-Ala-L-Val-L-Leu-L-Gln + 4-nitroaniline 3.4.22.69 SARS coronavirus main proteinase

MeOSuc-Ala-Ala-Ala-Arg_4-nitroanilide + H2O <=> MeOSuc-Ala-Ala-Ala-Arg + 4-nitroaniline 3.4.21.4 trypsin

MeOSuc-Ala-Ala-Arg_4-nitroanilide + H2O <=> MeOSuc-Ala-Ala-Arg + 4-nitroaniline 3.4.21.4 trypsin

MeOSuc-Ala-Arg_4-nitroanilide + H2O <=> MeOSuc-Ala-Arg + 4-nitroaniline 3.4.21.4 trypsin

Ac-YVAD-4-nitroanilide + H2O <=> acetyl-YVAD + 4-nitroaniline 3.4.22.36 caspase-1

Asp-Glu-Val-Asp-4-nitroanilide + H2O <=> DEVD + 4-nitroaniline 3.4.22.56 caspase-3

beta-Ala-Gly-Arg-4-nitroanilide + H2O <=> beta-Ala-Gly-Arg + 4-nitroaniline 3.4.21.5 thrombin

Glu-Ala-Leu-Phe-Gln-4-nitroanilide + H2O <=> Glu-Ala-Leu-Phe-Gln + 4-nitroaniline 3.4.22.28 picornain 3C

Ile-Glu-Thr-Asp-4-nitroanilide + H2O <=> Ile-Glu-Thr-Asp + 4-nitroaniline 3.4.22.56 caspase-3
3.4.22.61 caspase-8


MAAPV-p-nitroanilide + H2O <=> MAAPV + 4-nitroaniline 3.4.21.37 leukocyte elastase

N-benzoyl-Arg-Arg-4-nitroanilide + H2O <=> Bz-Arg-Arg + 4-nitroaniline 3.4.22.1 cathepsin B

N-succinyl-Gly-Pro-Lys-p-nitroanilide + H2O <=> succinyl-Gly-L-Pro-L-Lys + 4-nitroaniline 3.4.21.83 oligopeptidase B

N-succinyl-Phe-Ala-Ala-Phe-p-nitroanilide + H2O <=> succinyl-Phe-Ala-Ala-Phe + 4-nitroaniline 3.4.24.B26

5-L-glutamyl-4-nitroanilide + L-glutamine <=> 4-nitroaniline + 5-L-Glutamyl-L-glutamine 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-alanine <=> 4-nitroaniline + gamma-glutamyl-L-alanine 2.3.2.2 gamma-glutamyltransferase

5-L-glutamyl-4-nitroanilide + L-phenylalanine <=> 4-nitroaniline + 5-L-Glutamyl-L-phenylalanine 2.3.2.2 gamma-glutamyltransferase

L-Lys-L-Pro-p-nitroanilide + H2O <=> Lys-Pro + 4-nitroaniline 3.4.14.2 dipeptidyl-peptidase II
3.4.14.5 dipeptidyl-peptidase IV


L-Lys-L-Pro-p-nitroanilide + H2O <=> Gly-Pro + 4-nitroaniline 3.4.14.5 dipeptidyl-peptidase IV

D-Leu-Phg-Arg-4-nitroanilide + H2O <=> D-Leu-Phg-Arg + 4-nitroaniline 3.4.21.22 coagulation factor IXa

LETD-4-nitroanilide + H2O <=> LETD + 4-nitroaniline 3.4.22.61 caspase-8

methoxysuccinyl-Ala-Ile-Pro-Met-4-nitroanilide + H2O <=> methoxysuccinyl-Ala-Ile-Pro-Met + 4-nitroaniline 3.4.21.62 subtilisin

H2O + Aspartic_acid-p-nitroanilide <=> 4-nitroaniline + L-aspartate 3.4.11.10 bacterial leucyl aminopeptidase
3.4.11.21 aspartyl aminopeptidase


H2O + benzoyl-L-arginine-p-nitroanilide <=> N-Benzoyl-arginine + 4-nitroaniline 3.4.21.10 acrosin
3.4.21.59 tryptase